Anti-Prosurfactant Protein B antibody (ab101804)


  • Product name
    Anti-Prosurfactant Protein B antibody
    See all Prosurfactant Protein B primary antibodies
  • Description
    Rabbit polyclonal to Prosurfactant Protein B
  • Specificity
    Detects full length Prosurfactant Protein B (42 kDa). Is also expected to bind to mature form (aa 201-279, ~9 kDa).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Prosurfactant Protein B aa 200-300 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab116184)

  • Positive control
    • This antibody gave a positive signal in both WI38 and A549 whole cell lysates.



Our Abpromise guarantee covers the use of ab101804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).


  • Function
    Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
  • Involvement in disease
    Pulmonary surfactant metabolism dysfunction 1
    Respiratory distress syndrome in premature infants
  • Sequence similarities
    Contains 1 saposin A-type domain.
    Contains 3 saposin B-type domains.
  • Cellular localization
    Secreted, extracellular space, surface film.
  • Information by UniProt
  • Database links
  • Alternative names
    • 18 kDa pulmonary-surfactant protein antibody
    • 6 kDa protein antibody
    • PSP B antibody
    • PSPB antibody
    • PSPB_HUMAN antibody
    • Pulmonary surfactant apoprotein PSP-B antibody
    • Pulmonary surfactant-associated protein B antibody
    • Pulmonary surfactant-associated protein, 18-KD antibody
    • Pulmonary surfactant-associated proteolipid SPL(Phe) antibody
    • SFTB3 antibody
    • SFTP3 antibody
    • SFTPB antibody
    • SMDP1 antibody
    • SP B antibody
    • SP-B antibody
    • SPB antibody
    • surfactant protein B antibody
    • Surfactant, pulmonary-associated protein B antibody
    • surfactant-associated protein, pulmonary, 3 antibody
    see all

Anti-Prosurfactant Protein B antibody images

  • All lanes : Anti-Prosurfactant Protein B antibody (ab101804) at 1 µg/ml

    Lane 1 : WI-38 whole cell lysate (ab3960)
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 39 kDa (possible cleavage fragment).

    Exposure time : 16 minutes

    The band observed at 39 kDa could potentially be a cleaved form of Prosurfactant Protein B due to the presence of a 24 amino acid signal peptide. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References for Anti-Prosurfactant Protein B antibody (ab101804)

ab101804 has not yet been referenced specifically in any publications.

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