Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Prosurfactant Protein B aa 200-300 conjugated to Keyhole Limpet Haemocyanin (KLH). (Peptide available as ab116184)
This antibody gave a positive signal in both WI38 and A549 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
FunctionPulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
Involvement in diseasePulmonary surfactant metabolism dysfunction 1 Respiratory distress syndrome in premature infants
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa Observed band size : 42 kDa Additional bands at : 39 kDa (possible cleavage fragment).
Exposure time : 16 minutes
The band observed at 39 kDa could potentially be a cleaved form of Prosurfactant Protein B due to the presence of a 24 amino acid signal peptide. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
References for Anti-Prosurfactant Protein B antibody (ab101804)
has not yet been referenced specifically in any publications.
Publishing research using ab101804? Please let us know so that we can cite the reference in this datasheet.
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