The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
Use at an assay dependent concentration. PubMed: 23350749
Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
Involvement in disease
Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'.
Immunocytochemistry/ Immunofluorescence - Anti-Prosurfactant Protein C antibody (ab40879)Image from Akram KM et al., Respiratory Research. 2013;14:9:. Fig 1.; doi: 10.1186/1465-9921-14-9: Published by BioMed Central
ab40879 staining Prosurfactant Protein C in A549 cells from Human lung tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were incubated with primary antibody (1/250). A FITC-conjugated anti-rabbit (1/200) was used as the secondary antibody. DAPI was used to detect the nucleus of the cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody (ab40879)Image from Raredon et al, BioResearch Open Access, 1, 72-83, Fig. 3e; doi: 10.1089/biores.2016.0006
Immunohistochemical analysis of Pig lung tissue, labeling prosurfactant protein C with ab40879. Tissue fixed in 10% buffered formalin for 4 hours, and paraffin embedded. Anitgen retrieval performed in buffer at pH 9 at 75°C for 20 minutes. Blocked with PBS, treated with primary antibody, followed by secondary, and DAPI stained.
Immunohistochemistry (Paraffin-embedded sections) - Prosurfactant Protein C antibody (ab40879)
Adult mouse lung was embedded in paraffin and stained with anti-N terminal proSP-C rabbit serum ab40879 (1/2000 dilution).
Surolia R et al. 3D pulmospheres serve as a personalized and predictive multicellular model for assessment of antifibrotic drugs. JCI Insight2:e91377 (2017).
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