The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
Use at an assay dependent concentration. PubMed: 23350749
FunctionPulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
Involvement in diseaseDefects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'.
Sequence similaritiesContains 1 BRICHOS domain.
Cellular localizationSecreted > extracellular space > surface film.
SFTPC surfactant pulmonary associated protein C antibody
SP 5 antibody
SP C antibody
Surfactant associated protein pulmonary 2 antibody
Surfactant protein c antibody
Surfactant proteolipid SPL-pVal antibody
Surfactant pulmonary associated protein C antibody
Anti-Prosurfactant Protein C antibody images
Immunocytochemistry/ Immunofluorescence - Anti-Prosurfactant Protein C antibody (ab40879)Image from Akram KM et al., Respiratory Research. 2013;14:9:. Fig 1.; doi: 10.1186/1465-9921-14-9: Published by BioMed Central
ab40879 staining Prosurfactant Protein C in A549 cells from Human lung tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were incubated with primary antibody (1/250). A FITC-conjugated anti-rabbit (1/200) was used as the secondary antibody. DAPI was used to detect the nucleus of the cells.
Immunohistochemistry (Paraffin-embedded sections) - Prosurfactant Protein C antibody (ab40879)
Adult mouse lung was embedded in paraffin and stained with anti-N terminal proSP-C rabbit serum ab40879 (1/2000 dilution).
Western blot - Prosurfactant Protein C antibody (ab40879)
Anti-Prosurfactant Protein C antibody (ab40879) at 1/500 dilution + Lung (Human) Tissue Lysate at 10 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 21 kDa Observed band size : 21 kDa Additional bands at : 38 kDa,49 kDa,79 kDa. We are unsure as to the identity of these extra bands.
Akram KM et al. Alveolar epithelial cells in idiopathic pulmonary fibrosis display upregulation of TRAIL, DR4 and DR5 expression with simultaneous preferential over-expression of pro-apoptotic marker p53. Int J Clin Exp Pathol7:552-64 (2014).
Read more (PubMed: 24551275) »