Recombinant
RabMAb

Anti-Protamine 2 antibody [EPR15738] (ab190791)

Overview

  • Product name
    Anti-Protamine 2 antibody [EPR15738]
  • Description
    Rabbit monoclonal [EPR15738] to Protamine 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Protamine 2 aa 1-100. The exact sequence is proprietary.
    Database link: P04554

  • Positive control
    • Human testis lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15738
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab190791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).

Target

  • Relevance
    Protamines are small, arginine-rich proteins involved in the condensation of sperm chromatin. During spermiogenesis, histones are replaced by protamines (P1 and P2), resulting in sperm chromatin condensation followed by a halt to gene expression in haploid spermatids and spermatozoa. As a consequence, protamine deficiency and aberrant P1/P2 ratio have a profound effect on both fertilization and embryo development.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • Basic nuclear protein HPI1 antibody
    • Basic nuclear protein HPI2 antibody
    • Basic nuclear protein HPS1 antibody
    • Basic nuclear protein HPS2 antibody
    • Cancer/testis antigen family 94 member 2 antibody
    • CT94.2 antibody
    • PRM2 antibody
    • Protamine P2 HP2 antibody
    • Sperm histone HP2 antibody
    • Sperm histone HP3 antibody
    • Sperm histone HP4 antibody
    • Sperm histone P2 antibody
    • Sperm protamine P2 antibody
    • Sperm protamine P3 antibody
    • Sperm protamine P4 antibody
    see all

Images

  • Anti-Protamine 2 antibody [EPR15738] (ab190791) at 1/10000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 13 kDa
    Observed band size: 17 kDa (why is the actual band size different from the predicted?)

References

ab190791 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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