Overview

  • Product nameProtease Activity Assay Kit
    See all Protease kits
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Sensitivity
    < 500 pg/well
  • Assay time
    1h 00m
  • Product overview

    Abcam's Protease Activity Assay Kit is designed for the quantitative determination of proteases present in the protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-Casein is highly quenched. Upon digestion by proteases present in the sample the FITC-Casein substrate is cleaved into smaller peptides which abolishes the quenching of the fluorescence label. The fluorescence of the FITC-labeled peptide fragments is measured at Ex/Em = 485/530 nm. The kit is supplied with our Mass Spectrometry Grade (MSG), chemically stabilized Trypsin for use as a general protease control. However, other protease standard controls can also be used. This kit is easy to use and can detect < 500 pg/well Trypsin present in the sample
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Proteases are naturally present in all organisms. These enzymes are involved in a multitude of physiological reactions from simple digestion of food proteins to highly regulated cascades. Proteases can either break specific peptide bonds (limited proteolysis), depending on the amino acid sequence of a protein, or break down a complete peptide to amino acids (unlimited proteolysis). The activity can be a destructive change (abolishing a protein's function), an activation of a function (preform to mature form) or it can be a signal in a signaling pathway.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab111750 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent dilution.

    Protease Activity Assay Kit images

    • Example Standard curve obtained using ab111750
    • Positive control assayed using ab111750

    Protocols

    References for Protease Activity Assay Kit (ab111750)

    ab111750 has not yet been referenced specifically in any publications.

    Product Wall

    Sonication can build heat and affect protease activity. However, mild sonication (40-50% intensity) for 1-3 seconds each pulse for 10-12 pulses while keeping the sample on ice might work. Ideally we recommend a glass dounce homogenizer since it is effe...

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    Thank you for contacting Abcam.

    We are not sure about preserving samples in RNAlater at -80 for ab111750 as we have not tested this, but yes, flash freezing in liquid nitrogen and then storing at -80 should be fine. I will not recommend storin...

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    Thank you very much for your reply.

    I am checking with the lab regarding the upper limit of the kits, and I will get back to you as soon as I have that information.

    I might recommend the kit ab111750 out of these three, as it has be...

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    Thank you very much for contacting us with your question.

    We have only tested these kits with biological samples at this time, and we do not have information about their use with environmental samples. Could you tell me a little bit more abou...

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    Thank you for your enquiry and your interest in our products.
    This kit detects the total protease activity.
    If you need any further assistance in the future, please do not hesitate to contact me.

    Thank you again for your patience while I have been in touch with the lab regarding these kits.

    The kit ab111750 should not be used with turbid or non-clear samples, but my contact at the lab did say that ab112152 or ab112153 might be ok to u...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"