Overview

  • Product nameProtease Inhibitor Cocktail
  • Product overview

    Abcam's Protease Inhibitor Cocktail contains a mixture of water-soluble protease inhibitors with broad specificity for various proteases, and has been optimized to generate the maximal protection to mammalian tissue and cell extracts.

    Form:

    Supplied as a lyophilized powder.

    One vial can make 125 ml of cocktail solution.

    Preparation:

    Add 250 µl DMSO to the vial, pipette several times to dissolve all powder (This makes 500X concentrated inhibitor cocktail).


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Crude cell extracts contain many endogeneous enzymes, such as proteases and phosphotases, which can degrade valuable proteins in the extract. The individual components of the protease inhibitor cocktail have specific inhibitory properties. A description of each inhibitor is given below:

    Aprotinin: Inhibits serine proteases, such as trypsin, chymotrypsin, plasmin, trypsinogen, urokinase, and kallikrein.

    Leupeptin: Inhibits both serine and cysteine proteases, such as calpain, trypsin, papain, and cathepsin B.

    Pepstain A: Inhibits acid proteases, such as pepsin, rennin, cathepsin D, chymosin, and protease B.

    PMSF: Inhibits serine proteases, such as trypsin and chymotrypsin. Also inhibits cysteine proteases and mammalian acetylcholinesterase.

     

Properties

  • RelevanceCrude cell extracts contain many endogeneous enzymes, such as proteases and phosphotases, which can degrade valuable proteins in an extract. A protease inhibitor cocktail contains a mixture of protease inhibitors with broad specificity for various proteases.
  • Alternative names
    • Aprotinin
    • Leupeptin
    • Pepstatin
    • PMSF

References for Protease Inhibitor Cocktail (ab65621)

This product has been referenced in:
  • Wang B  et al. Essential control of mitochondrial morphology and function by chaperone-mediated autophagy through degradation of PARK7. Autophagy 12:1215-28 (2016). Read more (PubMed: 27171370) »
  • Filous AR  et al. Entrapment via synaptic-like connections between NG2 proteoglycan+ cells and dystrophic axons in the lesion plays a role in regeneration failure after spinal cord injury. J Neurosci 34:16369-84 (2014). Read more (PubMed: 25471575) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us.  To reconstitute this product, add 250-µl DMSO to the vial and pipet several times to dissolve all powder (This makes 500X concentrated inhibitor cocktail).  I have added these instructions to our datasheet.  I hope this he...

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I can confirm that ab65621 Protease Inhibitor Cocktail can be used on saliva samples.


I can confirm that ab65621 Protease Inhibitor Cocktail contains EDTA.

The protease inhibitor cocktail can be stored in small aliquots at -20C after reconstitution in DMSO for 1 year. We recommend to avoid freezing and thawing during this time.


After reconstituting in DMSO, the cocktail should be stored at -20C.


The laboratory said you can reconstitute ab65621 in 250 µl of DMSO. This makes it 250 µl of 500X inhibitor cocktail. Which means that for every 500 µl of your sample can add 1 µl of the inhibitor. These reconstitution inst...

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Thank you for contacting us.


The alternatives are as follows

1. ab103746; http://www.abcam.com/BrightMount-25ml-Aqueous-Mounting-Medium-for-Fluorescent-Staining-ab103746.html

or ab128982; http://www.abcam.com/Aqueous-Mou...

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Thank you for contacting us. To the best of our knowledge the SDS is compliant with Canadian legislation as long as it has the statement This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations (...

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Thank you for contacting us.

Could you please provide a catalog number for this product? I did find that we do carry a Protease Inhibitors and have attached the SDS from our datasheet for that product (ab65621) however if this is not the c...

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This product is provided as a lyophilized powder. You have to reconstitute it in 250 µl of DMSO. This makes it 250 µl of 500X inhibitor cocktail. Which means that for every 499 or 500 µl of the lysate you can add 1 µl of the inh...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"