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Other Immunogen Type corresponding to Proteasome 19S S4. Yeast proteasome 19S subunit S4.
Our Abpromise guarantee covers the use of ab3317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/1000. PubMed: 18986984|
|WB||1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 49 kDa).
This antibody detects a 56 kDa protein representing proteasome 19S subunit S4 in HeLa cell lysate. This antibody detects, to a lesser extent, an ~35 kDa protein which could correspond to subunit degradation product.
|IP||Use at an assay dependent concentration.|
Western blot of proteasome 19S subunit S4 on HeLa cell lysate using ab3317.
ab3317 staining Proteasome 19S S4 in mock transfected rat primary neurons by Immunocytochemistry/ Immunofluorescence. Primary cultures of rat hippocampal neurons were prepared from E18 rats. Cells were fixed in 4% paraformaldehyde and incubated with primary antibody at 1/1000 dilution. The image show red staining with ab3317, nucleus was stained blue with Hoechst H33258 and right image show merged images. The green color seen in image is the GFP staining in similar experiment.