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Rabbit polyclonal to Proteasome 19S S5A
HumanPredicted to work with:
Mouse, Chicken, Cow, Dog, Pig, Xenopus laevis, Monkey, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 80-319 of Human Proteasome 19S S5A (Uniprot ID: P55036).
- A549, HeLa, HepG2 and HCT116 whole cell lysates; A549 cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 40 kDa.
||1/100 - 1/1000.
Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
Belongs to the proteasome subunit S5A family.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
Contains 1 VWFA domain.
The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
- 26S protease subunit S5a antibody
- 26S proteasome non ATPase regulatory subunit 4 antibody
- 26S proteasome non-ATPase regulatory subunit 4 antibody
Western blot - Anti-Proteasome 19S S5A antibody (ab154935)
Anti-Proteasome 19S S5A antibody (ab154935) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 19S S5A antibody (ab154935)
Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labeling Proteasome 19S S5A with ab154935 at 1/200 dilution (green). Lower image shows cells co-stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"