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Western blot - Proteasome 19S S6' antibody (ab3321)
(enlarge)
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Product Name
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Proteasome 19S S6' antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Proteasome 19S S6'
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
(species key)
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Hu
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Specificity
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Detects proteasome 19S subunit S6'.
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Western Blot: use at 1/1000.
By Western blot, this antibody detects a 48 kDa protein representing proteasome 19S subunit S6' from HeLa cell lysate.
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Research areas
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Cell Biology >> Other Antibodies >> Oxidative Stress
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Proteasome
Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
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Relevance
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Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes and the endoplasmic reticulum.
The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman’s syndrome and Liddle syndrome.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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PBS, 1 mg/ml BSA, 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Proteasome 19S S6' antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Proteasome 19S S6' antibody images, references, products related to ab3321 and other tools.
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Proteasome 19S S6' antibody images:
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 Western blot - Proteasome 19S S6' antibody (ab3321)
Western blot on partially purified human 19S proteasome using ab3321.
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Search PubMed (MEDLINE) for references to Proteasome 19S S6'
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Proteasome 19S S6' antibody - more information
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Customer reviews (feedback) regarding Proteasome 19S S6' antibody |
Customer FAQs regarding Proteasome 19S S6' antibody |
Protocols for Proteasome 19S S6' antibody |
Price and availability of products related to Proteasome 19S S6' antibody |
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Proteasome 19S S6' antibody - related products:
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Proteasome 19S S6' antibody - other tools:
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Contact Abcam with a Technical Enquiry about Proteasome 19S S6' antibody |
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