Anti-Proteasome 19S S7 antibody (ab49058)

Overview

  • Product nameAnti-Proteasome 19S S7 antibody
    See all Proteasome 19S S7 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome 19S S7
  • SpecificityThis antibody is specific for Proteasome 19S S7
  • Tested applicationsSuitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: MPDYLGADQR KTKEDEKDDK PIRALDEGDI ALLKTYGQST YSRQIKQVED, corresponding to N terminal amino acids 1-50 of Human Proteasome 19S S7

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionThe 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, positive modulator of Tat-mediated transactivation.
  • Sequence similaritiesBelongs to the AAA ATPase family.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 26S protease complex antibody
    • 26S protease regulatory subunit 7 antibody
    • 26S proteasome AAA-ATPase subunit RPT1 antibody
    • mammalian suppressor of sgv-1 of yeast antibody
    • MSS1 antibody
    • Nbla10058 antibody
    • protease 26S subunit 7 antibody
    • proteasome 26S ATPase subunit 2 antibody
    • Proteasome 26S subunit ATPase 2 antibody
    • Proteasome 26S subunit ATPase 2 antibody
    • Protein MSS1 antibody
    • PRS7_HUMAN antibody
    • PSMC2 antibody
    • putative protein product of Nbla10058 antibody
    • S7 antibody
    see all

Anti-Proteasome 19S S7 antibody images

  • All lanes : Anti-Proteasome 19S S7 antibody (ab49058) at 1.25 µg/ml

    Lane 1 : MARKER
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 49 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

References for Anti-Proteasome 19S S7 antibody (ab49058)

ab49058 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Rat Cell (brain tissue IHC-Fr)
Specification brain tissue IHC-Fr
Permeabilization Yes - tritonx-100
Fixative Paraformaldehyde
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Abcam user community

Verified customer

Submitted May 24 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (brain)
Loading amount 25 µg
Specification brain
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted May 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"