Proteasome 20S Activity Assay Kit (Fluorometric) (ab112154)

Overview

  • Product nameProteasome 20S Activity Assay Kit (Fluorometric)
  • Tests
    1 x 96 well plate
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab112154 Proteasome 20S Activity Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex in cultured cells. ab112154 uses LLVY-R110 as a fluorogenic indicator for proteasome activities. Cleavage of LLVY-R110 by the proteasome generates strongly green fluorescent R110 that is monitored fluorometrically at 520-530 nm with excitation at 480-500 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays to evaluate the proteasome activities or screen inhibitors in cultured cells or in solution. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format.

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  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab112154 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Proteasome 20S Activity Assay Kit (Fluorometric) images

    • Detection of Proteasome Activity in Jurkat cells. Jurkat cells were seeded on the same day at 500,000 cells/90 µL/well in a 96-well black wall/clear bottom plate. The cells were treated with or without 50 mM H2O2 for 30 minutes. The proteasome assay loading solution (100 µL/well) was added and incubated in a 5% CO2, 37 °C incubator for 3 hours. The fluorescence intensity was measured at Ex/Em = 490/525.

    Protocols

    References for Proteasome 20S Activity Assay Kit (Fluorometric) (ab112154)

    ab112154 has not yet been referenced specifically in any publications.

    Product Wall

    Merci pour votre requête. J'ai confirmé avec le laboratoire et je peux vous confirmer que les deux kits le ab107921 et le ab112154, fonctionnent sur du tissue de rat. Nous pouvons donc vous garantir ces kits aussi pour la mesure de l'activité de protea...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"