Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)

Overview

  • Product nameAnti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231]
  • Description
    Mouse monoclonal [MCP231] to Proteasome 20S alpha 1+2+3+5+6+7
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, ELISAmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, Saccharomyces cerevisiae, Potato
    Predicted to work with: a wide range of other species
  • Immunogen

    Full length protein (dinitrophenylated proteasomes).

  • EpitopeReacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to alpha type subunits, although not necessarily identical in all. In general this motif is phylogenetically preserved.
  • Positive control
    • Human placental proteasome.
  • General notesDilute antibody to working dilution in PBS , pH 7.2 - 7.4. Store at 4°C and use within 1 month. Do not freeze.


    This antibody reacts with six different alpha type subunits:- alpha 1, 2, 3, 5 , 6 and 7.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01M Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityAscites
  • Primary antibody notesThis antibody reacts with six different alpha type subunits:- alpha 1, 2, 3, 5 , 6 and 7.
  • ClonalityMonoclonal
  • Clone numberMCP231
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA Use at an assay dependent concentration.
  • Application notesIs unsuitable for IP.
  • Target

    Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] images

    • ICC/IF image of ab22674 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22674, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Overlay histogram showing HepG2 cells stained with ab22674 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22674, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References for Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)

    This product has been referenced in:

    See all 4 Publications for this product

    Product Wall

    Application IHC - Wholemount
    Sample Drosophila melanogaster Tissue (Eye)
    Specification Eye
    Username

    Mr. Lee Sun

    Verified customer

    Submitted Oct 25 2016

    Application Western blot
    Sample Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions Reduced Denaturing (15%)
    Loading amount 20 µg
    Specification Skeletal muscle
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 19 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions Reduced Denaturing (15%)
    Loading amount 20 µg
    Specification Skeletal muscle
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 19 2016

    Application Immunocytochemistry/ Immunofluorescence
    Sample Dog Cell (Canine Mammary tumor cell (CMT-28))
    Permeabilization No
    Specification Canine Mammary tumor cell (CMT-28)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative Methanol
    Username

    Miss. Samantha Guillemette

    Verified customer

    Submitted Nov 25 2015

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing
    Sample Xenopus laevis Cell lysate - whole cell (Egg extracts)
    Specification Egg extracts
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 22 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Skeletal muscle)
    Loading amount 30 µg
    Specification Skeletal muscle
    Gel Running Conditions Reduced Denaturing (15% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. G.K Sakellariou

    Verified customer

    Submitted May 16 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Cell (cochlea)
    Specification cochlea
    Fixative paraformaldehyde + glutaraldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 30 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (cytoplasmic fraction placental villous tissue)
    Specification cytoplasmic fraction placental villous tissue
    Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Aug 11 2005

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"