Anti-Proteasome 20S alpha 2 antibody [EPR5453] (ab109525)


  • Product name
    Anti-Proteasome 20S alpha 2 antibody [EPR5453]
    See all Proteasome 20S alpha 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR5453] to Proteasome 20S alpha 2
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 2 aa 50-150.
    Database link: P25787

  • Positive control
    • HepG2, 293T, HeLa or JAR cell lysate; Human brain tissue; HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab109525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
    • Sequence similarities
      Belongs to the peptidase T1A family.
    • Post-translational
      Phosphorylated on tyrosine residues; which may be important for nuclear import.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • HC3 antibody
      • Macropain subunit C3 antibody
      • MU antibody
      • Multicatalytic endopeptidase complex subunit C3 antibody
      • PMSA2 antibody
      • Proteasome (prosome macropain) subunit alpha type 2 antibody
      • Proteasome alpha 2 subunit antibody
      • Proteasome component C3 antibody
      • Proteasome subunit alpha type 2 antibody
      • Proteasome subunit alpha type-2 antibody
      • Proteasome subunit HC3 antibody
      • PSA2_HUMAN antibody
      • PSC2 antibody
      • PSC3 antibody
      • PSMA 2 antibody
      • psmA2 antibody
      see all

    Anti-Proteasome 20S alpha 2 antibody [EPR5453] images

    • All lanes : Anti-Proteasome 20S alpha 2 antibody [EPR5453] (ab109525) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size : 25 kDa
    • ab109525, at a 1/250 dilution, staining Human Proteasome 20S alpha 2 in brain tissue, using Immunohistochemistry, (Formalin/PFA-fixed paraffin-embedded).
    • ab109525, at 1/50 dilution, staining Proteasome 20S alpha 2 in HeLa cells by Immunofluorescence.

    References for Anti-Proteasome 20S alpha 2 antibody [EPR5453] (ab109525)

    This product has been referenced in:
    • Wang S  et al. Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury. Oncotarget 7:17681-98 (2016). Read more (PubMed: 26769846) »
    • Pla A  et al. TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome pathways induced by ethanol treatment in brain. Cell Death Dis 5:e1066 (2014). WB ; Mouse . Read more (PubMed: 24556681) »

    See all 2 Publications for this product

    Product Wall

    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12 %)
    Human Cell lysate - whole cell (HaCaT human immortalized keratinocyte cell line, 3)
    HaCaT human immortalized keratinocyte cell line, 3
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Iva Doleckova

    Verified customer

    Submitted May 31 2014

    Thank you for your inquiry. Regarding ab109525, we have not actually measured non-IgG protein concentration. The only known non-IgG protein in our antibody is BSA (0.05%). Please contact us for the specific IgG concentration of the lot you received...

    Read More


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