Recombinant Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (ab109502)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5454] to Proteasome 20S alpha 2/HC3
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454]
See all Proteasome 20S alpha 2/HC3 primary antibodies -
Description
Rabbit monoclonal [EPR5454] to Proteasome 20S alpha 2/HC3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, 293T, HeLa or JAR cell lysate. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5454 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 26 kDa. |
Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation. -
Sequence similarities
Belongs to the peptidase T1A family. -
Post-translational
modificationsPhosphorylated on tyrosine residues; which may be important for nuclear import. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5683 Human
- Entrez Gene: 19166 Mouse
- Entrez Gene: 29669 Rat
- Omim: 176842 Human
- SwissProt: P25787 Human
- SwissProt: P49722 Mouse
- SwissProt: P17220 Rat
- Unigene: 333786 Human
see all -
Alternative names
- HC3 antibody
- Macropain subunit C3 antibody
- MU antibody
see all
Images
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All lanes : Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (ab109502) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : JAR cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa -
Intracellular flow cytometric analysis of permeabilized HeLa cells using ab109502 at 1/10 dilution (red) and a control rabbit IgG (green).
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab109502 has been referenced in 2 publications.
- Zhao WT et al. The Yunnan national medicine Maytenus compound inhibits the proliferation of hepatocellular carcinoma (HCC) by suppressing the activation of the EGFR-PI3K-AKT signaling pathway. J Cancer 12:3325-3334 (2021). PubMed: 33976742
- Niu Y et al. Huaier Suppresses the Hepatocellular Carcinoma Cell Cycle by Regulating Minichromosome Maintenance Proteins. Onco Targets Ther 13:12015-12025 (2020). PubMed: 33244243