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Product name Anti-Proteasome 20S alpha 2 antibody [EPR5454] See all Proteasome 20S alpha 2 primary antibodies
Rabbit monoclonal [EPR5454] to Proteasome 20S alpha 2
Tested applications Suitable for:
WB, Flow Cyt more details Unsuitable for:
ICC,IHC-P or IP
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 2 aa 200-300.
Database link: P25787
WB: Jurkat, 293T, HeLa or JAR cell lysate.
FC: HeLa cells.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR5454
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
1/10 - 1/100.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notes Is unsuitable for ICC,IHC-P or IP.
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
Sequence similarities Belongs to the peptidase T1A family.
Post-translational modifications Phosphorylated on tyrosine residues; which may be important for nuclear import.
Cellular localization Cytoplasm. Nucleus.
Information by UniProt
Macropain subunit C3 antibody
Anti-Proteasome 20S alpha 2 antibody [EPR5454] images
Western blot - Proteasome 20S alpha 2 antibody [EPR5454] (ab109502)
All lanes : Anti-Proteasome 20S alpha 2 antibody [EPR5454] (ab109502) at 1/10000 dilution Lane 1 : Jurkat cell lysate Lane 2 : 293T cell lysate Lane 3 : HeLa cell lysate Lane 4 : JAR cell lysates Lysates/proteins at 10 µg per lane. Secondary Standard HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size : 26 kDa
Flow Cytometry - Proteasome 20S alpha 2 antibody [EPR5454] (ab109502)
Flow cytometric analysis of permeabilized HeLa cells using ab109502 at 1/10 dilution (red) and a control rabbit IgG (green).
References for Anti-Proteasome 20S alpha 2 antibody [EPR5454] (ab109502)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"