Anti-Proteasome 20S alpha 2 antibody [EPR5454] (ab109502)

Overview

  • Product nameAnti-Proteasome 20S alpha 2 antibody [EPR5454]
    See all Proteasome 20S alpha 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR5454] to Proteasome 20S alpha 2
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 2 aa 200-300.
    Database link: P25787

  • Positive control
    • WB: Jurkat, 293T, HeLa or JAR cell lysate. FC: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
    • Sequence similaritiesBelongs to the peptidase T1A family.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues; which may be important for nuclear import.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • HC3 antibody
      • Macropain subunit C3 antibody
      • MU antibody
      • Multicatalytic endopeptidase complex subunit C3 antibody
      • PMSA2 antibody
      • Proteasome (prosome macropain) subunit alpha type 2 antibody
      • Proteasome alpha 2 subunit antibody
      • Proteasome component C3 antibody
      • Proteasome subunit alpha type 2 antibody
      • Proteasome subunit alpha type-2 antibody
      • Proteasome subunit HC3 antibody
      • PSA2_HUMAN antibody
      • PSC2 antibody
      • PSC3 antibody
      • PSMA 2 antibody
      • psmA2 antibody
      see all

    Anti-Proteasome 20S alpha 2 antibody [EPR5454] images

    • All lanes : Anti-Proteasome 20S alpha 2 antibody [EPR5454] (ab109502) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : JAR cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 26 kDa
    • Flow cytometric analysis of permeabilized HeLa cells using ab109502 at 1/10 dilution (red) and a control rabbit IgG (green).

    References for Anti-Proteasome 20S alpha 2 antibody [EPR5454] (ab109502)

    ab109502 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"