Anti-Proteasome 20S alpha 2 antibody [MCP21] (ab22666)

Overview

  • Product nameAnti-Proteasome 20S alpha 2 antibody [MCP21]
    See all Proteasome 20S alpha 2 primary antibodies
  • Description
    Mouse monoclonal [MCP21] to Proteasome 20S alpha 2
  • Specificityab22666 reacts with 20S proteasome subunit a2 (HC3).
  • Tested applicationsSuitable for: Flow Cyt, WB, IP, Othermore details
  • Species reactivity
    Reacts with: Rabbit, Human
    Does not react with: Mouse, Rat
  • Immunogen

    Full length protein corresponding to Human Proteasome 20S alpha 2.
    Database link: P25787

  • Positive control
    • human placental proteasome preparation and HeLa cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01M Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numberMCP21
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 27 kDa.
IP Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
Other Use at an assay dependent concentration.

Target

  • FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
  • Sequence similaritiesBelongs to the peptidase T1A family.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues; which may be important for nuclear import.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HC3 antibody
    • Macropain subunit C3 antibody
    • MU antibody
    • Multicatalytic endopeptidase complex subunit C3 antibody
    • PMSA2 antibody
    • Proteasome (prosome macropain) subunit alpha type 2 antibody
    • Proteasome alpha 2 subunit antibody
    • Proteasome component C3 antibody
    • Proteasome subunit alpha type 2 antibody
    • Proteasome subunit alpha type-2 antibody
    • Proteasome subunit HC3 antibody
    • PSA2_HUMAN antibody
    • PSC2 antibody
    • PSC3 antibody
    • PSMA 2 antibody
    • psmA2 antibody
    see all

Anti-Proteasome 20S alpha 2 antibody [MCP21] images

  • Luminograph of human placental proteasome preparation after 2D PADE followed by blotting onto nitrocellulose and probing with ab22666 (1/1000). ECL exposure , 1 minute exposure).
  • All lanes : Anti-Proteasome 20S alpha 2 antibody [MCP21] (ab22666) at 1/1000 dilution

    Lane 1 : 40ug SU-DHL1 whole cell lysate
    Lane 2 : 40ug Karpas 299 whole cell lysate
    Lane 3 : 40ug JB-6 whole cell lysate
    Lane 4 : 40ug SUP-M2 whole cell lysate
    Lane 5 : 40ug Mac-1 whole cell lysate
    Lane 6 : 40ug Jurkat whole cell lysate

    Secondary
    Biotinylated polyclonal anti-mouse antibody.
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 27 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Carlo Vigliani

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab22666 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22666, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Proteasome 20S alpha 2 antibody [MCP21] (ab22666)

This product has been referenced in:
  • Juenemann K  et al. Expanded polyglutamine-containing N-terminal huntingtin fragments are entirely degraded by mammalian proteasomes. J Biol Chem 288:27068-84 (2013). Read more (PubMed: 23908352) »

See 1 Publication for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (T cell lymphoma)
Loading amount 40 µg
Specification T cell lymphoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Carlo Vigliani

Verified customer

Submitted Sep 07 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"