Anti-Proteasome 20S alpha 3 antibody [EPR5455] (ab109532)

Overview

  • Product nameAnti-Proteasome 20S alpha 3 antibody [EPR5455]
    See all Proteasome 20S alpha 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5455] to Proteasome 20S alpha 3
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 3 aa 50-150.
    Database link: P25788

  • Positive control
    • A549, JAR, PC12 and NIH3T3 cell lysates; Human brain tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
    • Sequence similaritiesBelongs to the peptidase T1A family.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • HC8 antibody
      • Macropain subunit C8 antibody
      • MGC12306 antibody
      • MGC32631 antibody
      • Multicatalytic endopeptidase complex subunit C8 antibody
      • Proteasome (prosome macropain) subunit alpha type 3 antibody
      • Proteasome alpha 3 subunit antibody
      • Proteasome component C8 antibody
      • Proteasome subunit alpha type 3 antibody
      • Proteasome subunit alpha type-3 antibody
      • Proteasome subunit C8 antibody
      • PSA3_HUMAN antibody
      • PSC8 antibody
      • psmA3 antibody
      see all

    Anti-Proteasome 20S alpha 3 antibody [EPR5455] images

    • All lanes : Anti-Proteasome 20S alpha 3 antibody [EPR5455] (ab109532) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 28 kDa
    • ab109532, at 1/100 dilution, staining Proteasome 20S alpha 3 in paraffin embedded Human brain tissue by Immunohistochemistry.
    • ab109532, at 1/100 dilution, staining Proteasome 20S alpha 3 in HeLa cells by Immunofluorescence.

    References for Anti-Proteasome 20S alpha 3 antibody [EPR5455] (ab109532)

    ab109532 has not yet been referenced specifically in any publications.

    Product Wall

    We do not have any data suggesting that the antibody has higher affinity for nuclear PSMA3 than for cytoplasmic PSMA3. The differences in staining intensity may be due to unequal distribution of the protein (more PSMA3 in the nucleus than in the cytopl...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"