Anti-Proteasome 20S alpha 3 (phospho S250) antibody (ab79184)

Overview

  • Product name
    Anti-Proteasome 20S alpha 3 (phospho S250) antibody
    See all Proteasome 20S alpha 3 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome 20S alpha 3 (phospho S250)
  • Specificity
    Detects endogenous levels of Proteasome 20S alpha 3 only when phosphorylated at serine 250.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 20S alpha 3 (phospho S250).
    Database link: P25788

  • Positive control
    • Extracts from 3T3 cells, treated with EGF (200 ng/ml, 30 minutes)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.
ELISA 1/5000.

Target

  • Function
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
  • Sequence similarities
    Belongs to the peptidase T1A family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HC8 antibody
    • Macropain subunit C8 antibody
    • MGC12306 antibody
    • MGC32631 antibody
    • Multicatalytic endopeptidase complex subunit C8 antibody
    • Proteasome (prosome macropain) subunit alpha type 3 antibody
    • Proteasome alpha 3 subunit antibody
    • Proteasome component C8 antibody
    • Proteasome subunit alpha type 3 antibody
    • Proteasome subunit alpha type-3 antibody
    • Proteasome subunit C8 antibody
    • PSA3_HUMAN antibody
    • PSC8 antibody
    • psmA3 antibody
    see all

Images

  • All lanes : Anti-Proteasome 20S alpha 3 (phospho S250) antibody (ab79184) at 1/500 dilution

    Lane 1 : Extracts from 3T3 cells, treated with EGF (200 ng/ml, 30 minutes)
    Lane 2 : Extracts from 3T3 cells, treated with EGF (200 ng/ml, 30 minutes) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 28 kDa

References

ab79184 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab79184.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up