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Rabbit polyclonal to Proteasome 20S alpha 3 (phospho S250)
Detects endogenous levels of Proteasome 20S alpha 3 only when phosphorylated at serine 250.
Predicted to work with:
Synthetic peptide corresponding to Human Proteasome 20S alpha 3 (phospho S250).
Database link: P25788
Extracts from 3T3 cells, treated with EGF (200 ng/ml, 30 minutes)
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Affinity purified from rabbit antiserum by affinity chromatography using
epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 28 kDa.
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
Belongs to the peptidase T1A family.
Information by UniProt
Macropain subunit C8 antibody
Western blot - Proteasome 20S alpha 3 (phospho S250) antibody (ab79184)
All lanes : Anti-Proteasome 20S alpha 3 (phospho S250) antibody (ab79184) at 1/500 dilution Lane 1 : Extracts from 3T3 cells, treated with EGF (200 ng/ml, 30 minutes) Lane 2 : Extracts from 3T3 cells, treated with EGF (200 ng/ml, 30 minutes) with immunizing peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size: 28 kDa Observed band size: 28 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"