Our Abpromise guarantee covers the use of ab11437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 26.5 kDa (predicted molecular weight: 26.5 kDa).Can be blocked with Human Proteasome 20S alpha 5 peptide (ab41791).|