Overview

  • Product nameAnti-Proteasome 20S alpha 5 antibody
    See all Proteasome 20S alpha 5 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome 20S alpha 5
  • Tested applicationsSuitable for: IHC-P, ICC/IF, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human
    Predicted to work with: Cow, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 20S alpha 5 aa 120-133.
    Sequence:

    ALQFGEEDADPGAM


    Database link: P28066
    (Peptide available as ab41791)

  • Positive control
    • Hela cell extract/cell line slide.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26.5 kDa (predicted molecular weight: 26.5 kDa).Can be blocked with Human Proteasome 20S alpha 5 peptide (ab41791).

Target

Anti-Proteasome 20S alpha 5 antibody images

  • All lanes : Anti-Proteasome 20S alpha 5 antibody (ab11437)

    Lane 1 : HeLa cell extract.
    Lane 2 : HeLa cell extract with blocking peptide.


    Predicted band size : 26.5 kDa
    Observed band size : 26.5 kDa
  • Immunofluorescence of HeLa cells using ab11437.
  • IHC image of ab11437 staining in human liver carcinoma liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11437, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Proteasome 20S alpha 5 antibody (ab11437)

This product has been referenced in:
  • Freudenburg W  et al. Reduction in ATP levels triggers immunoproteasome activation by the 11S (PA28) regulator during early antiviral response mediated by IFNß in mouse pancreatic ß-cells. PLoS One 8:e52408 (2013). Mouse . Read more (PubMed: 23383295) »
  • Ohta A  et al. Herpes simplex virus type 1 UL14 tegument protein regulates intracellular compartmentalization of major tegument protein VP16. Virol J 8:365 (2011). ICC/IF . Read more (PubMed: 21791071) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us for technical support with ab11437, I'm sorry to hear you are experiencing problems with this antibody. Could you please clarify the type of cells in culture that you used? We have not tested ab11437 on whole sections, on...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"