For the best experience on the Abcam website please upgrade to a modern browser such as
Product name Anti-Proteasome 20S alpha 5 antibody [EPR5832] See all Proteasome 20S alpha 5 primary antibodies
Rabbit monoclonal [EPR5832] to Proteasome 20S alpha 5
Tested applications Suitable for:
WB, IHC-P, ICC, Flow Cyt more details Unsuitable for:
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 5 (N terminal).
Database link: P28066
WB: HepG2, 293T and JAR cell lysates.
IHC-P: Human testis tissue
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K D)
K D = 3.22 x 10
Learn more about K D
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR5832
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
1/25 - 1/50.
1/10 - 1/100.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notes Is unsuitable for IP.
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Tissue specificity Expressed in fetal brain (at protein level).
Sequence similarities Belongs to the peptidase T1A family.
Cellular localization Cytoplasm. Nucleus.
Information by UniProt
Macropain zeta chain antibody
Multicatalytic endopeptidase complex zeta chain antibody
Proteasome (prosome macropain) subunit alpha type 5 antibody
Anti-Proteasome 20S alpha 5 antibody [EPR5832] images
Western blot - Proteasome 20S alpha 5 antibody [EPR5832] (ab109387)
All lanes : Anti-Proteasome 20S alpha 5 antibody [EPR5832] (ab109387) at 1/1000 dilution Lane 1 : HepG2 cell lysates Lane 2 : 293T cell lysates Lane 3 : JAR cell lysates Lysates/proteins at 10 µg per lane. Predicted band size : 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteasome 20S alpha 5 antibody [EPR5832] (ab109387)
Immunohistochemical analysis of paraffin-embedded Human testis tissue using 1/100 ab109387.
Other-Anti-Proteasome 20S alpha 5 antibody [EPR5832](ab109387)
References for Anti-Proteasome 20S alpha 5 antibody [EPR5832] (ab109387)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"