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Rabbit monoclonal [EPR5832] to Proteasome 20S alpha 5
Flow Cyt more details Unsuitable for:
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 5 (N terminal).
Database link: P28066
WB: HepG2, 293T and JAR cell lysates.
IHC-P: Human testis tissue
This product is a recombinant rabbit monoclonal antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
1/25 - 1/50.
1/10 - 1/100.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IP.
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Expressed in fetal brain (at protein level).
Belongs to the peptidase T1A family.
Information by UniProt
Macropain zeta chain antibody
Multicatalytic endopeptidase complex zeta chain antibody
Proteasome (prosome macropain) subunit alpha type 5 antibody
Western blot - Proteasome 20S alpha 5 antibody [EPR5832] (ab109387)
All lanes : Anti-Proteasome 20S alpha 5 antibody [EPR5832] (ab109387) at 1/1000 dilution Lane 1 : HepG2 cell lysates Lane 2 : 293T cell lysates Lane 3 : JAR cell lysates Lysates/proteins at 10 µg per lane. Predicted band size : 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteasome 20S alpha 5 antibody [EPR5832] (ab109387)
Immunohistochemical analysis of paraffin-embedded Human testis tissue using 1/100 ab109387.
Other-Anti-Proteasome 20S alpha 5 antibody [EPR5832](ab109387)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"