Anti-Proteasome 20S alpha + beta antibody (ab22673)
Key features and details
- Rabbit polyclonal to Proteasome 20S alpha + beta
- Suitable for: IP, WB, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 20S alpha + beta antibody -
Description
Rabbit polyclonal to Proteasome 20S alpha + beta -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae -
Immunogen
Tissue/ cell preparation: Proteasomal preparation isolated from human red blood cells.
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General notes
Dilute to working concentration in PBS pH 7.2- 7.4, and store at 2 to 4°C (do not freeze). Use diluted antibody within 1 month.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB | (2) |
1/1000.
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IHC-Fr | (1) |
Use at an assay dependent concentration.
|
ICC/IF | (4) |
Use at an assay dependent concentration.
See Abreview. PubMed: 23840376 |
Notes |
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IP
Use at an assay dependent concentration. |
WB
1/1000. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. See Abreview. PubMed: 23840376 |
Target
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Relevance
The proteasome is widely recognised as the central enzyme of non lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP dependent pathway of protein degradation. The catalytic core of this unusually large (2000 kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, alpha and beta, each group having distinct structural and functional roles. The alpha subunits comprise the outer rings and the beta subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (alpha7 beta7 beta7 alpha7) with a member of each sub family represented in each particle. Each subunit is located in a unique position within the alpha or beta rings. 20S Proteasomes degrade only unfolded proteins in an energy independent manner, whereas 26S proteasomes degrade native and ubiquitinylated proteins in an ATP dependent manner. The native protein substrates are recognised by subunits, some with ATP binding sites, of the outer 19S caps of the 26S proteasome. -
Cellular localization
Cytoplasmic and Nuclear. -
Database links
- Entrez Gene: 5686 Human
- Entrez Gene: 5688 Human
- Entrez Gene: 5689 Human
- Entrez Gene: 5693 Human
- Entrez Gene: 5694 Human
- Entrez Gene: 5695 Human
- Entrez Gene: 19170 Mouse
- Entrez Gene: 19173 Mouse
see all -
Alternative names
- Proteasome subunit alpha type 1 antibody
- Proteasome subunit alpha type 5 antibody
- Proteasome subunit alpha type 7 antibody
see all
Images
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ab22673 staining human Hela cells by ICC/IF. Cells were formaldehyde fixed, permeabilized in 0.1% Triton and blocked in 3% BSA for 15 minutes at room temperature. The primary antibody was diluted 1/2000 (3% BSA) and incubated with sample for 1 hour. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit, diluted 1/1000 was used as secondary.
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Immunofluorescence analysis of HeLa cells, staining Proteasome 20S alpha + beta with ab22673.
Cells were fixed with formaldehyde and permeabilized using 0.1% Triton X-100. Samples were incubated with primary antibody (1/1000) fr 1 hour at room temperature. An AlexaFluor®555-conjugated donkey anti-rabbit IgG was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab22673 has been referenced in 36 publications.
- Ibañez-Vega J et al. Ecm29-Dependent Proteasome Localization Regulates Cytoskeleton Remodeling at the Immune Synapse. Front Cell Dev Biol 9:650817 (2021). PubMed: 34055780
- Yunusova NV et al. Exosomal Protease Cargo as Prognostic Biomarker in Colorectal Cancer. Asian Pac J Cancer Prev 22:861-869 (2021). PubMed: 33773551
- Chen L et al. Coordinated regulation of the ribosome and proteasome by PRMT1 in the maintenance of neural stemness in cancer cells and neural stem cells. J Biol Chem 297:101275 (2021). PubMed: 34619150
- Yunusova N et al. Plasma Exosomes of Patients with Breast and Ovarian Tumors Contain an Inactive 20S Proteasome. Molecules 26:N/A (2021). PubMed: 34834058
- Gomes MJ et al. Effects of aerobic and resistance exercise on cardiac remodelling and skeletal muscle oxidative stress of infarcted rats. J Cell Mol Med 24:5352-5362 (2020). PubMed: 32239667