Anti-Proteasome 20S alpha + beta antibody (ab22673)

Overview

  • Product nameAnti-Proteasome 20S alpha + beta antibody
  • Description
    Rabbit polyclonal to Proteasome 20S alpha + beta
  • SpecificityThis antibody detects a combination of alpha and beta proteasome subunits. For example in western blotting of purified 20S proteasome, subunits correlating to a5/a7, ß1, ß5, ß5i, ß7 can be identified.
  • Tested applicationsSuitable for: ICC, IHC-Fr, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae
  • Immunogen

    Tissue/ cell preparation: Proteasomal preparation isolated from human red blood cells.

  • Positive control
    • Human and yeast 20S proteasome and tissue/cell preparations including human placental proteasome preparation, a HeLa cell lysate, RSV 3T3 mouse fibroblast cell lysate, and yeast whole cell extract.
  • General notesDilute to working concentration in PBS pH 7.2- 7.4, and store at 2 to 4°C (do not freeze). Use diluted antibody within 1 month.

Properties

Applications

Our Abpromise guarantee covers the use of ab22673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration. PubMed: 23840376
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 25 - 30 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. See Abreview.

Target

Anti-Proteasome 20S alpha + beta antibody images

  • Anti-Proteasome 20S alpha + beta antibody (ab22673) at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 25 - 30 kDa
    Observed band size : 25,26,28 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
  • All lanes : Anti-Proteasome 20S alpha + beta antibody (ab22673) at 1/2000 dilution

    Lane 1 : Whole tissue lysate prepared from rat prefrontal cortex
    Lane 2 : Whole tissue lysate prepared from rat prefrontal cortex
    Lane 3 : Whole tissue lysate prepared from rat prefrontal cortex
    Lane 4 : Whole tissue lysate prepared from rat prefrontal cortex
    Lane 5 : Whole tissue lysate prepared from rat prefrontal cortex

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-rabbit IgG-HRP at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 25 - 30 kDa


    Exposure time : 5 minutes

    This image is courtesy of an anonymous abreview.

    Blocked with 5% milk for 30 minutes at 27°C.

    See Abreview

  • ab22673 staining human Hela cells by ICC/IF. Cells were formaldehyde fixed, permeabilized in 0.1% Triton and blocked in 3% BSA for 15 minutes at room temperature. The primary antibody was diluted 1/2000 (3% BSA) and incubated with sample for 1 hour. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit, diluted 1/1000 was used as secondary.

    See Abreview

  • Immunofluorescence analysis of HeLa cells, staining Proteasome 20S alpha + beta with ab22673.

    Cells were fixed with formaldehyde and permeabilized using 0.1% Triton X-100. Samples were incubated with primary antibody (1/1000) fr 1 hour at room temperature. An AlexaFluor®555-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

References for Anti-Proteasome 20S alpha + beta antibody (ab22673)

This product has been referenced in:
  • Schipper-Krom S  et al. Dynamic recruitment of active proteasomes into polyglutamine initiated inclusion bodies. FEBS Lett 588:151-9 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24291262) »
  • Yang HY  et al. Entecavir combined with furin inhibitor simultaneously reduces hepatitis B virus replication and e antigen secretion. Virol J 11:165 (2014). WB . Read more (PubMed: 25224377) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Human Cell (HS 5)
Specification HS 5
Permeabilization Yes - Triton X 100
Fixative Formaldehyde
Username

Mr. Kannan Govindaraj

Verified customer

Submitted Aug 02 2013

Thank you for contacting us yesterday.

I am sorry but as you are aware we do not currently have any of the Anti-Proteasome 20S alpha + beta antibody (ab22673). As discussed over the phone, I have had a look for antibodies which may serve as su...

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Thank you for your reply.

I can change the antibody on your current order from ab22673 for ab109525 if you would like. As this is in stock, if you let me know you would like for me to do this, it can be with you by tomorrow.

The ant...

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Thank you for contacting us and your interest in our products.

Unfortunately, we do not have a direct alternative for the Anti-Proteasome 20S alpha + beta antibody (ab22673). This antibody detects a combination of alpha and beta proteasome su...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (kidney)
Loading amount 20 µg
Specification kidney
Gel Running Conditions Reduced Denaturing (4-12 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 11 2012

Thank you for your reply.

The credit note ID is ***.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our Finance department to issue a credit note for you. The credit note may be used in one ...

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Thank you for your reply and for sending the additional information.

I would be happyto issue a credit note for the customer. Do you have the order number or PO number that this was ordered on? I found one going to Interlab from February 7, o...

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Thank you for contacting us and forwarding the customer's protocol details.

I do have a few additional questions, so that I better understand the situation.

The secondary is listed as HRP conjugated, and the detection system is list...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Prefrontal cortex)
Loading amount 20 µg
Specification Prefrontal cortex
Gel Running Conditions Non-reduced Denaturing (10% acrylamide)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Nov 11 2011

DISCOUNT CODE: *** Expiration date: February 20th, 2012 I am very pleased to hear you would like to accept our offer and test ab22673 in rat. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"