Anti-Proteasome 20S beta 3 antibody (ab88665)

Overview

  • Product nameAnti-Proteasome 20S beta 3 antibody
    See all Proteasome 20S beta 3 primary antibodies
  • Description
    Mouse polyclonal to Proteasome 20S beta 3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Proteasome 20S beta 3.
    Database link: P49720

  • Positive control
    • Human liver tissue lysate. Proteasome 20S beta 3 transfected 293T cell lysate. HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
  • Sequence similaritiesBelongs to the peptidase T1B family.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C10 II antibody
    • HC10 II antibody
    • proteasome (prosome, macropain) subunit, beta type 3 antibody
    • Proteasome beta 3 subunit antibody
    • Proteasome chain 13 antibody
    • proteasome component C10 II antibody
    • Proteasome component C10-II antibody
    • Proteasome subunit beta type-3 antibody
    • Proteasome theta chain antibody
    • PSB3_HUMAN antibody
    • PSMB3 antibody
    see all

Anti-Proteasome 20S beta 3 antibody images

  • Anti-Proteasome 20S beta 3 antibody (ab88665) at 1 µg/ml + human liver tissue lysate at 50 µg

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
  • All lanes : Anti-Proteasome 20S beta 3 antibody (ab88665) at 1 µg/ml

    Lane 1 : Proteasome 20S beta 3 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • ab88665, at 10 µg/ml, staining Proteasome 20S beta 3 in Hela cells by Immunofluorescence.

References for Anti-Proteasome 20S beta 3 antibody (ab88665)

ab88665 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"