Anti-Proteasome 20S beta 7 antibody (ab154745)

Overview

  • Product name
    Anti-Proteasome 20S beta 7 antibody
    See all Proteasome 20S beta 7 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome 20S beta 7
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to Human Proteasome 20S beta 7 aa 85-277.
    Database link: Q99436

  • Positive control
    • A549, HeLa, HepG, HCT116 and mouse brain lysates; HeLa cells; Human MDA-MB157 xenograft.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
  • Tissue specificity
    Expressed at a low level in colonic mucosa. Up-regulated in colorectal cancer tissues.
  • Sequence similarities
    Belongs to the peptidase T1B family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Macropain chain Z antibody
    • Multicatalytic endopeptidase complex chain Z antibody
    • Proteasome (prosome macropain) subunit beta type 7 antibody
    • Proteasome beta 7 subunit antibody
    • Proteasome catalytic subunit 2 antibody
    • Proteasome subunit alpha antibody
    • Proteasome subunit beta 7 antibody
    • Proteasome subunit beta type-7 antibody
    • Proteasome subunit Z antibody
    • PSB7_HUMAN antibody
    • PSMB7 antibody
    • PUP1 antibody
    • Z antibody
    see all

Images

  • Anti-Proteasome 20S beta 7 antibody (ab154745) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 30 kDa
  • Anti-Proteasome 20S beta 7 antibody (ab154745) at 1/1000 dilution + mouse brain whole cell lysate at 50 µg

    Predicted band size : 30 kDa
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Proteasome 20S beta 7 with ab154745 at 1/200 dilution. Lower panel was co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft labeling Proteasome 20S beta 7 with ab154745 at 1/100 dilution.

References

ab154745 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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