Anti-Proteasome 20S beta 7 antibody (ab3332)

Overview

  • Product name
    Anti-Proteasome 20S beta 7 antibody
    See all Proteasome 20S beta 7 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome 20S beta 7
  • Specificity
    Detects proteasome 20S beta 7 (also known as the beta 2 subunit) from purified bovine and human 26S proteasome samples.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Cow, Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 20S beta 7 aa 263-277 (N terminal).
    Sequence:

    EIEVLEETVQTMDTS


    Database link: Q99436
    (Peptide available as ab4948)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 90 kDa).Can be blocked with Proteasome 20S beta 7 peptide (ab4948).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
  • Tissue specificity
    Expressed at a low level in colonic mucosa. Up-regulated in colorectal cancer tissues.
  • Sequence similarities
    Belongs to the peptidase T1B family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Macropain chain Z antibody
    • Multicatalytic endopeptidase complex chain Z antibody
    • Proteasome (prosome macropain) subunit beta type 7 antibody
    • Proteasome beta 7 subunit antibody
    • Proteasome catalytic subunit 2 antibody
    • Proteasome subunit alpha antibody
    • Proteasome subunit beta 7 antibody
    • Proteasome subunit beta type-7 antibody
    • Proteasome subunit Z antibody
    • PSB7_HUMAN antibody
    • PSMB7 antibody
    • PUP1 antibody
    • Z antibody
    see all

Images



  • Predicted band size : 90 kDa

    Western blot of proteasome 20S beta 7 in purified human 26S proteasome samples using ab3332.

References

ab3332 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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