The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Detects a band of approximately 65 kDa (predicted molecular weight: 30 kDa).
We recommend using about 2.5 ug of positive control protein in the colorimetric method, and about 10X less for ECL detection.
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
Expressed at a low level in colonic mucosa. Up-regulated in colorectal cancer tissues.