Anti-Proteasome 20S beta 7 antibody (ab37616)

Overview

  • Product nameAnti-Proteasome 20S beta 7 antibody
    See all Proteasome 20S beta 7 primary antibodies
  • Description
    Chicken polyclonal to Proteasome 20S beta 7
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 20S beta 7 aa 151-277 (C terminal).
    Database link: Q99436

  • Positive control
    • Proteasome 20S beta2 fusion protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab37616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 65 kDa (predicted molecular weight: 30 kDa).

We recommend using about 2.5 ug of positive control protein in the colorimetric method, and about 10X less for ECL detection.

Target

  • FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
  • Tissue specificityExpressed at a low level in colonic mucosa. Up-regulated in colorectal cancer tissues.
  • Sequence similaritiesBelongs to the peptidase T1B family.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Macropain chain Z antibody
    • Multicatalytic endopeptidase complex chain Z antibody
    • Proteasome (prosome macropain) subunit beta type 7 antibody
    • Proteasome beta 7 subunit antibody
    • Proteasome catalytic subunit 2 antibody
    • Proteasome subunit alpha antibody
    • Proteasome subunit beta 7 antibody
    • Proteasome subunit beta type-7 antibody
    • Proteasome subunit Z antibody
    • PSB7_HUMAN antibody
    • PSMB7 antibody
    • PUP1 antibody
    • Z antibody
    see all

Anti-Proteasome 20S beta 7 antibody images

  • Anti-Proteasome 20S beta 7 antibody (ab37616) at 1/2000 dilution + E coli derived fusion protein

    Secondary
    Goat anti IgY HRP at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 30 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

References for Anti-Proteasome 20S beta 7 antibody (ab37616)

ab37616 has not yet been referenced specifically in any publications.

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