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Our Abpromise guarantee covers the use of ab3325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Can be blocked with Human Proteasome 20S C2 peptide (ab4943).|
|IHC-P||1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
Western blot of 20S C2 subunit in CHO cell extract using ab3325.