Mouse, Rat, Human Predicted to work with:
Cow, Zebrafish, Rainbow Trout, Leaf frog (Phyllomedusa), Orangutan, Zebra finch
Synthetic peptide corresponding to Human Proteasome 20S C2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH). Database link: P25786 (Peptide available as ab93073, ab93073)
This antibody gave a positive signal in the following lysates: HeLa whole cell; HepG2 whole cell; Jurkat whole cell; MCF7 whole cell; PC3 whole cell; HEK293 whole cell; human liver tissue; mouse heart tissue; rat heart tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
Sequence similaritiesBelongs to the peptidase T1A family.