Anti-Proteasome 20S C2 antibody [EPR5451] (ab109500)

Overview

  • Product nameAnti-Proteasome 20S C2 antibody [EPR5451]
    See all Proteasome 20S C2 primary antibodies
  • Description
    Rabbit monoclonal [EPR5451] to Proteasome 20S C2
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S C2 aa 1-100.
    Database link: P25786

  • Positive control
    • HeLa, Jurkat, HepG2, and PC-3 cell lysates Human breast tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR5451
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform antigen retrieval
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
    • Sequence similaritiesBelongs to the peptidase T1A family.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 30 kDa prosomal protein antibody
      • HC 2 antibody
      • HC2 antibody
      • Macropain subunit C2 antibody
      • Macropain subunit nu antibody
      • MGC14542 antibody
      • MGC14575 antibody
      • MGC14751 antibody
      • MGC1667 antibody
      • MGC21459 antibody
      • MGC22853 antibody
      • MGC23915 antibody
      • Multicatalytic endopeptidase complex subunit C2 antibody
      • NU antibody
      • PROS 30 antibody
      • PROS-30 antibody
      • PROS30 antibody
      • Proteasome (prosome macropain) subunit alpha type 1 antibody
      • Proteasome alpha 1 subunit antibody
      • Proteasome component C2 antibody
      • Proteasome nu chain antibody
      • Proteasome subunit alpha type 1 antibody
      • Proteasome subunit alpha type I antibody
      • Proteasome subunit alpha type-1 antibody
      • Proteasome subunit nu antibody
      • Protein P30 33K antibody
      • PSA1_HUMAN antibody
      • PSC 2 antibody
      • PSC2 antibody
      • PSMA 1 antibody
      • psmA1 antibody
      see all

    Anti-Proteasome 20S C2 antibody [EPR5451] images

    • Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109500 at 1/250 dilution.
    • All lanes : Anti-Proteasome 20S C2 antibody [EPR5451] (ab109500) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : PC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 30 kDa

    References for Anti-Proteasome 20S C2 antibody [EPR5451] (ab109500)

    ab109500 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109500.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"