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Product name Anti-Proteasome 20S C2 antibody [EPR5451] See all Proteasome 20S C2 primary antibodies
Rabbit monoclonal [EPR5451] to Proteasome 20S C2
Tested applications Suitable for:
WB, IP, IHC-P, ICC more details Unsuitable for:
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S C2 aa 1-100.
Database link: P25786
HeLa, Jurkat, HepG2, and PC-3 cell lysates
Human breast tissue
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR5451
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
1/10 - 1/100.
1/250 - 1/500. Perform antigen retrieval
1/100 - 1/250.
Application notes Is unsuitable for Flow Cyt.
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
Sequence similarities Belongs to the peptidase T1A family.
Cellular localization Cytoplasm. Nucleus.
Information by UniProt
30 kDa prosomal protein antibody
HC 2 antibody
Anti-Proteasome 20S C2 antibody [EPR5451] images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteasome 20S C2 antibody [EPR5451] (ab109500)
Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109500 at 1/250 dilution.
Western blot - Proteasome 20S C2 antibody [EPR5451] (ab109500)
All lanes : Anti-Proteasome 20S C2 antibody [EPR5451] (ab109500) at 1/1000 dilution Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : HepG2 cell lysate Lane 4 : PC-3 cell lysate Lysates/proteins at 10 µg per lane. Secondary Standard HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size : 30 kDa
References for Anti-Proteasome 20S C2 antibody [EPR5451] (ab109500)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"