The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
These peptides may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and mouse proteasome 20S LMP2, catalog ab4944. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~ 70-fold) for competitive inhibition of antibody-protein binding reactions.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Large multifunctional peptidase 2
Large multifunctional protease 2
Low molecular mass protein 2
Macropain chain 7
Multicatalytic endopeptidase complex chain 7
Proteasome (prosome macropain) subunit beta type 9
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
Sequence similaritiesBelongs to the peptidase T1B family.
Developmental stageHighly expressed in immature dendritic cells (at protein level).
Post-translational modificationsAutocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.