Overview

  • Product nameAnti-Proteasome 20S LMP7 antibody
    See all Proteasome 20S LMP7 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome 20S LMP7
  • SpecificityDetects proteasome 20S LMP7.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 20S LMP7 aa 259-274.
    Sequence:

    VESTDVSDLLHQYREA


    Database link: P28062
    (Peptide available as ab4945)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa.Can be blocked with Human Proteasome 20S LMP7 peptide (ab4945).
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr 1/500. PubMed: 17008387

Target

  • FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency.
  • Involvement in diseaseDefects in PSMB8 are the cause of JMP syndrome (JMPS) [MIM:613732]; also called joint contractures muscular atrophy microcytic anemia and panniculitis-induced lipodystrophy. JBTS1 is an autoinflammatory disorder characterized by childhood onset of joint stiffness and severe contractures of the hands and feet, erythematous skin lesions with subsequent development of severe lipodystrophy, and laboratory evidence of immune dysregulation. Accompanying features include muscle weakness and atrophy, hepatosplenomegaly, and microcytic anemia.
  • Sequence similaritiesBelongs to the peptidase T1B family.
  • Developmental stageHighly expressed in immature dendritic cells (at protein level).
  • Post-translational
    modifications
    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALDD antibody
    • D6S216 antibody
    • D6S216E antibody
    • Large multifunctional peptidase 7 antibody
    • Large multifunctional protease 7 antibody
    • LMP 7 antibody
    • LMP7 antibody
    • Low molecular mass protein 7 antibody
    • Low molecular weight protein 7 antibody
    • Macropain subunit C13 antibody
    • MGC1491 antibody
    • Multicatalytic endopeptidase complex subunit C13 antibody
    • NKJO antibody
    • OTTHUMP00000062981 antibody
    • Protease component C13 antibody
    • Proteasome (prosome macropain) subunit beta type 8 antibody
    • Proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) antibody
    • Proteasome beta 8 subunit antibody
    • Proteasome catalytic subunit 3i antibody
    • Proteasome component C13 antibody
    • Proteasome related gene 7 antibody
    • Proteasome subunit beta 5i antibody
    • Proteasome subunit beta 8 antibody
    • Proteasome subunit beta type 8 antibody
    • Proteasome subunit beta type antibody
    • Proteasome subunit beta type-8 antibody
    • Proteasome subunit beta-5i antibody
    • Proteasome subunit Y2 antibody
    • PSB8_HUMAN antibody
    • PSMB 8 antibody
    • PSMB5i antibody
    • PSMB8 antibody
    • Really interesting new gene 10 protein antibody
    • RING 10 antibody
    • RING10 antibody
    • Y2 antibody
    see all

Anti-Proteasome 20S LMP7 antibody images

  • Western blot of proteasome 20S LMP7 from HeLa cell extract using ab3329.
  • Ab3329 staining Human normal skin. Staining is localized to cytoplasmic and nuclear compartments.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required
  • ICC/IF image of ab3329 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3329, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Proteasome 20S LMP7 antibody (ab3329)

This product has been referenced in:
  • Chen X  et al. Inhibition of immunoproteasome reduces infarction volume and attenuates inflammatory reaction in a rat model of ischemic stroke. Cell Death Dis 6:e1626 (2015). ICC/IF ; Rat . Read more (PubMed: 25633295) »
  • Pla A  et al. TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome pathways induced by ethanol treatment in brain. Cell Death Dis 5:e1066 (2014). WB ; Mouse . Read more (PubMed: 24556681) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Feb 29 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 29 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Loading amount 30 µg
Specification Skeletal muscle
Gel Running Conditions Reduced Denaturing (15% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

Submitted May 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Recombinant LMP7-GST)
Loading amount 0.025 µg
Specification Recombinant LMP7-GST
Gel Running Conditions Reduced Denaturing (4-12% bis/tris)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted May 31 2012

Thank you for sending me this image. This data will be used to keep records of how our products are performing and for internal invetigation if needed. I noted that all tissue lysates that you have used have given the same non-specific signal. However ...

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Thank you for taking time to send us your protocols. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring pro...

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Thank you for contacting us. I would like to ask you if you might send me the protocols used with these productsso that we can gather further information regarding the samples tested and the protocol used. Once we receive your response, we will look at...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (dendritic cell)
Loading amount 1e+006 cells
Specification dendritic cell
Gel Running Conditions Non-reduced Denaturing
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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Verified customer

Submitted Jan 05 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Purified protein (heart and spleen)
Loading amount 1 µg
Specification heart and spleen
Gel Running Conditions Reduced Denaturing (12,5%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

Submitted Sep 03 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (D1 cells)
Loading amount 35 µg
Specification D1 cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Mar 06 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"