Anti-Proteasome 20S LMP7 antibody (ab82528)

Overview

  • Product nameAnti-Proteasome 20S LMP7 antibody
    See all Proteasome 20S LMP7 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome 20S LMP7
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig, Non Human Primates
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 20S LMP7 aa 250 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P28062
    (Peptide available as ab91637, ab91637)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lystes: Jurkat; Raji; U937.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 30 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency.
    • Involvement in diseaseDefects in PSMB8 are the cause of JMP syndrome (JMPS) [MIM:613732]; also called joint contractures muscular atrophy microcytic anemia and panniculitis-induced lipodystrophy. JBTS1 is an autoinflammatory disorder characterized by childhood onset of joint stiffness and severe contractures of the hands and feet, erythematous skin lesions with subsequent development of severe lipodystrophy, and laboratory evidence of immune dysregulation. Accompanying features include muscle weakness and atrophy, hepatosplenomegaly, and microcytic anemia.
    • Sequence similaritiesBelongs to the peptidase T1B family.
    • Developmental stageHighly expressed in immature dendritic cells (at protein level).
    • Post-translational
      modifications
      Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALDD antibody
      • D6S216 antibody
      • D6S216E antibody
      • Large multifunctional peptidase 7 antibody
      • Large multifunctional protease 7 antibody
      • LMP 7 antibody
      • LMP7 antibody
      • Low molecular mass protein 7 antibody
      • Low molecular weight protein 7 antibody
      • Macropain subunit C13 antibody
      • MGC1491 antibody
      • Multicatalytic endopeptidase complex subunit C13 antibody
      • NKJO antibody
      • OTTHUMP00000062981 antibody
      • Protease component C13 antibody
      • Proteasome (prosome macropain) subunit beta type 8 antibody
      • Proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) antibody
      • Proteasome beta 8 subunit antibody
      • Proteasome catalytic subunit 3i antibody
      • Proteasome component C13 antibody
      • Proteasome related gene 7 antibody
      • Proteasome subunit beta 5i antibody
      • Proteasome subunit beta 8 antibody
      • Proteasome subunit beta type 8 antibody
      • Proteasome subunit beta type antibody
      • Proteasome subunit beta type-8 antibody
      • Proteasome subunit beta-5i antibody
      • Proteasome subunit Y2 antibody
      • PSB8_HUMAN antibody
      • PSMB 8 antibody
      • PSMB5i antibody
      • PSMB8 antibody
      • Really interesting new gene 10 protein antibody
      • RING 10 antibody
      • RING10 antibody
      • Y2 antibody
      see all

    Anti-Proteasome 20S LMP7 antibody images

    • All lanes : Anti-Proteasome 20S LMP7 antibody (ab82528) at 1 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 3 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 30 kDa
      Observed band size : 22 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 145 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Proteasome 20S LMP7 antibody (ab82528)

    ab82528 has not yet been referenced specifically in any publications.

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