Overview

  • Product nameAnti-Proteasome 26S S10 antibody
    See all Proteasome 26S S10 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome 26S S10
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 143-192 (MHRAAAKGNL KMIHILLYYK ASTNIQDTEG NTPLHLACDE ERVEEAKLLV) of Human Proteasome 26S S10 (NP_002805).

  • Positive control
    • ACHN cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab98002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionActs as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module.
    Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export.
  • Tissue specificityOverexpressed in hepatocellular carcinomas.
  • Sequence similaritiesContains 7 ANK repeats.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 26S proteasome non-ATPase regulatory subunit 10 antibody
    • 26S proteasome regulatory subunit p28 antibody
    • Ankyrin repeat protein antibody
    • dJ889N15.2 antibody
    • Gankyrin antibody
    • Hepatocellular carcinoma-associated protein p28 II antibody
    • p28 antibody
    • p28(GANK) antibody
    • Proteasome (prosome, macropain) 26S subunit, non ATPase, 10 antibody
    • PSD10_HUMAN antibody
    • Psmd10 antibody
    see all

Anti-Proteasome 26S S10 antibody images

  • Anti-Proteasome 26S S10 antibody (ab98002) at 1 µg/ml (5% skim milk/ PBS buffer) + ACHN cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 24 kDa

References for Anti-Proteasome 26S S10 antibody (ab98002)

ab98002 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab98002.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"