Anti-Proteasome 26S S2/PSMD2 antibody (ab197054)
Key features and details
- Rabbit polyclonal to Proteasome 26S S2/PSMD2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 26S S2/PSMD2 antibody
See all Proteasome 26S S2/PSMD2 primary antibodies -
Description
Rabbit polyclonal to Proteasome 26S S2/PSMD2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment corresponding to Human Proteasome 26S S2/PSMD2 (N terminal).
Database link: Q13200 -
Positive control
- SW480, OVCAR3, M21, U937 and SHSYSY cell lysates. Mouse testis, heart and liver lysates. Human breast cancer and lung cancer tissues. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 100 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 100 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1. -
Tissue specificity
Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta. -
Sequence similarities
Belongs to the proteasome subunit S2 family.
Contains 7 PC repeats. - Information by UniProt
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Database links
- Entrez Gene: 5708 Human
- Entrez Gene: 21762 Mouse
- Entrez Gene: 287984 Rat
- Omim: 606223 Human
- SwissProt: Q13200 Human
- SwissProt: Q8VDM4 Mouse
- SwissProt: Q4FZT9 Rat
- Unigene: 518464 Human
see all -
Alternative names
- 26S proteasome non-ATPase regulatory subunit 2 antibody
- 26S proteasome regulatory subunit RPN1 antibody
- 26S proteasome regulatory subunit S2 antibody
see all
Images
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All lanes : Anti-Proteasome 26S S2/PSMD2 antibody (ab197054) at 1/500 dilution
Lane 1 : SW480 cell lysate
Lane 2 : OVCAR3 cell lysate
Lane 3 : M21 cell lysate
Lane 4 : U937 cell lysate
Lane 5 : SHSY5Y cell lysate
Lane 6 : mouse testis lysate
Lane 7 : mouse heart lysate
Lane 8 : mouse liver lysate
Predicted band size: 100 kDa -
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Proteasome 26S S2/PSMD2 with ab197054 at a 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Proteasome 26S S2/PSMD2 with ab197054 at a 1/200 dilution.
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Immunofluorescence analysis of HeLa cells, labeling Proteasome 26S S2/PSMD2 (green) with ab197054 at a 1/50 dilution. Blue: DAPI for nuclear staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab197054 has been referenced in 1 publication.
- Li Y et al. PSMD2 regulates breast cancer cell proliferation and cell cycle progression by modulating p21 and p27 proteasomal degradation. Cancer Lett 430:109-122 (2018). PubMed: 29777785