Anti-Proteasome 26S S3/PSMD3 antibody (ab140440)
Key features and details
- Rabbit polyclonal to Proteasome 26S S3/PSMD3
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 26S S3/PSMD3 antibody
See all Proteasome 26S S3/PSMD3 primary antibodies -
Description
Rabbit polyclonal to Proteasome 26S S3/PSMD3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Human Proteasome 26S S3/PSMD3 aa 1-100.
Database link: O43242 -
Positive control
- Recombinant Human Proteasome 26S S3/PSMD3 protein (ab117003) can be used as a positive control in WB. 293T, HeLa, Jurkat and NIH 3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab140440 was affinity purified using an epitope specific to Proteasome 26S S3/PSMD3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 61 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 61 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. -
Sequence similarities
Belongs to the proteasome subunit S3 family.
Contains 1 PCI domain. - Information by UniProt
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Database links
- Entrez Gene: 454640 Chimpanzee
- Entrez Gene: 504937 Cow
- Entrez Gene: 491018 Dog
- Entrez Gene: 100725725 Guinea pig
- Entrez Gene: 5709 Human
- Entrez Gene: 22123 Mouse
- Entrez Gene: 287670 Rat
- SwissProt: Q2KJ46 Cow
see all -
Alternative names
- 26S proteasome non ATPase regulatory subunit 3 antibody
- 26S proteasome non-ATPase regulatory subunit 3 antibody
- 26S proteasome regulatory subunit RPN3 antibody
see all
Images
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All lanes : Anti-Proteasome 26S S3/PSMD3 antibody (ab140440) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 30 seconds -
Detection of Proteasome 26S S3/PSMD3 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab140428 at 6 µg/mg lysate for IP and 0.4µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab140440 has not yet been referenced specifically in any publications.