Overview

  • Product name
    Anti-Proteasome 26S S3 antibody
    See all Proteasome 26S S3 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome 26S S3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-240 of Human Proteasome 26S S3 (O43242).

  • Positive control
    • A549, Hela, Jurkat, NT2D1, NIH 3T3 whole cell lysates; Cal27 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 89% PBS, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function
    Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • Sequence similarities
    Belongs to the proteasome subunit S3 family.
    Contains 1 PCI domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 26S proteasome non ATPase regulatory subunit 3 antibody
    • 26S proteasome non-ATPase regulatory subunit 3 antibody
    • 26S proteasome regulatory subunit RPN3 antibody
    • 26S proteasome regulatory subunit S3 antibody
    • OTTHUMP00000164347 antibody
    • P58 antibody
    • Proteasome (prosome, macropain) 26S subunit non ATPase 3 antibody
    • Proteasome subunit p58 antibody
    • PSMD3 antibody
    • PSMD3_HUMAN antibody
    • RPN3 antibody
    • S3 antibody
    • Tissue specific transplantation antigen 2 antibody
    • TSTA2 antibody
    see all

Images

  • Anti-Proteasome 26S S3 antibody (ab154963) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg/ml

    Predicted band size: 61 kDa



    7.5% SDS PAGE
  • Anti-Proteasome 26S S3 antibody (ab154963) at 1/500 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 61 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling Proteasome 26S S3 with ab154963 at 1/500.

References

ab154963 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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