Overview

  • Product nameAnti-Proteasome 26S S3 antibody
    See all Proteasome 26S S3 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome 26S S3
  • SpecificityDetects proteasome 26S subunit S3.
  • Tested applicationsSuitable for: ICC, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Non Human Primates
    Predicted to work with: Cow, Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome 26S S3 aa 513-534.
    Sequence:

    EREQQDLEFAKEMAEDDDDSFP

  • Positive control
    • HeLa cell extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab3316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).

For Western blot procedures it is necessary to transfer the protein to membrane at pH 11.

By Western blot, this antibody detects an ~60 kDa protein representing proteasome 19S subunit S3 from HeLa cell extract.

Target

  • FunctionActs as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • Sequence similaritiesBelongs to the proteasome subunit S3 family.
    Contains 1 PCI domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 26S proteasome non ATPase regulatory subunit 3 antibody
    • 26S proteasome non-ATPase regulatory subunit 3 antibody
    • 26S proteasome regulatory subunit RPN3 antibody
    • 26S proteasome regulatory subunit S3 antibody
    • OTTHUMP00000164347 antibody
    • P58 antibody
    • Proteasome (prosome, macropain) 26S subunit non ATPase 3 antibody
    • Proteasome subunit p58 antibody
    • PSMD3 antibody
    • PSMD3_HUMAN antibody
    • RPN3 antibody
    • S3 antibody
    • Tissue specific transplantation antigen 2 antibody
    • TSTA2 antibody
    see all

Anti-Proteasome 26S S3 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3 (green) showing staining in the cytoplasm and nucleus of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3 (green) showing staining in the cytoplasm and nucleus of HeLa cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

References for Anti-Proteasome 26S S3 antibody (ab3316)

ab3316 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab3316.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"