Overview

  • Product name
    Anti-Proteasome Activator Subunit 4 antibody
    See all Proteasome Activator Subunit 4 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome Activator Subunit 4
  • Tested applications
    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
    Predicted to work with: Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome Activator Subunit 4 aa 1620-1634.
    Sequence:

    TKLPKKRKRDPGSVG

  • Positive control
    • See "Application notes".

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/600.
IP Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Detects a band of approximately 200 kDa.

Target

Images

  • Anti-Proteasome Activator Subunit 4 antibody (ab5620) at 1/2000 dilution + Mouse testis tissue lysate at 25 µg

    Secondary
    HRP-conjugated anti-rabbit
    Developed using the ECL technique

    Observed band size : 160,200 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab5620 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Pickering AM & Davies KJ Differential roles of proteasome and immunoproteasome regulators Pa28aß, Pa28? and Pa200 in the degradation of oxidized proteins. Arch Biochem Biophys 523:181-90 (2012). WB ; Mouse . Read more (PubMed: 22564544) »
  • Greenhaff PL  et al. Disassociation between the effects of amino acids and insulin on signaling, ubiquitin ligases, and protein turnover in human muscle. Am J Physiol Endocrinol Metab 295:E595-604 (2008). WB ; Human . Read more (PubMed: 18577697) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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