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Rabbit monoclonal [EPR13576(B)] to Proteasome Activator Subunit 4 - N-terminal
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Proteasome Activator Subunit 4 aa 1-100. The exact sequence is proprietary.
Database link: Q14997
HepG2, 293T, Jurkat, HeLa and K562 lysates; Jurkat cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
Concentration information loading...
Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 211 kDa).
Activates proteasomal cleavage of peptides in an energy-independent manner. May be involved in spermatogenesis. May be involved in DNA repair.
Contains 6 HEAT repeats.
Nucleus. Nucleus speckle. Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2).
Information by UniProt
Proteasome Activator 200 kDa antibody
Flow Cytometry - Anti-Proteasome Activator Subunit 4 [EPR13576(B)] antibody (ab181128)
Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling Proteasome Activator Subunit 4 with ab181128 at 1/210 dilution (red) or a Rabbit monoclonal IgG (negative) (green) followed by Goat anti rabbit IgG (FITC) secondary used at 1/150 dilution.
Western blot - Anti-Proteasome Activator Subunit 4 [EPR13576(B)] antibody (ab181128)
All lanes : Anti-Proteasome Activator Subunit 4 antibody [EPR13576(B)] - N-terminal (ab181128) at 1/10000 dilution Lane 1 : HepG2 lysate Lane 2 : 293T lysate Lane 3 : Jurkat lysate Lane 4 : HeLa lysate Lane 5 : K562 lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution Predicted band size: 211 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"