Recombinant
RabMAb

Anti-Proteasome Activator Subunit 4 antibody [EPR13577(B)] - C-terminal (ab181203)

Overview

  • Product name
    Anti-Proteasome Activator Subunit 4 antibody [EPR13577(B)] - C-terminal
    See all Proteasome Activator Subunit 4 primary antibodies
  • Description
    Rabbit monoclonal [EPR13577(B)] to Proteasome Activator Subunit 4 - C-terminal
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Proteasome Activator Subunit 4 aa 1750 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14997

  • Positive control
    • HeLa and Jurkat cells; K562, HepG2 and HeLa cell lysates; Human pancreas and cardiac muscle tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR13577(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 200 kDa (predicted molecular weight: 211 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/110. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • All lanes : Anti-Proteasome Activator Subunit 4 antibody [EPR13577(B)] - C-terminal (ab181203) at 1/1000 dilution

    Lane 1 : K562 cell line lysate
    Lane 2 : HeLa cell line lysate
    Lane 3 : HepG2 cell line lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size : 211 kDa
  • Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Proteasome Activator Subunit 4 with ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling Proteasome Activator Subunit 4 with ab181203 at 1/110 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunofluorescent analysis of  HeLa cells (-20℃ Acetone-fixed) labeling Proteasome Activator Subunit 4 with ab181203 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution and counterstained with Dapi (blue).

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Proteasome Activator Subunit 4 with ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

References

ab181203 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181203.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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