Proteasome Activity Assay Kit (ab107921)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell Lysate
Overview
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Product name
Proteasome Activity Assay Kit -
Detection method
Fluorescent -
Sample type
Cell Lysate -
Assay type
Enzyme activity -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Proteasome Activity Assay Kit (ab107921) takes advantage of the chymotrypsin-like activity, using an AMC-tagged peptide substrate (Proteasome Substrate (Succ-LLVY-AMC in DMSO), which releases free, highly fluorescent AMC (Ex/Em 350/440 nm) in the presence of proteolytic activity.
The kit also includes a positive control (Jurkat Cell lysate with significant proteasome activity) and a specific proteasome inhibitor MG-132 which suppresses all proteolytic activity due to proteasomes. This permits differentiation of proteasome activity from other protease activity which may be present in samples.
Proteasome activity assay protocol summary:
- add samples, standards and positive control to wells
- add proteasome inhibitor to half of sample and positive control wells
- add proteasome substrate to sample and control wells
- incubate for 20 min
- analyze with microplate reader, incubate for further 30 min, analyze again -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K245 Proteasome Activity Fluorometric Assay Kit. K245-100 is the same size as the 100 test size of ab107921.
The 20S proteasome assembly is the functional protease structure with chymotrypsin-like, trypsin-like and caspase-like protease activities
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests AMC Standard 1 x 100µl Proteasome Assay Buffer 1 x 25ml Proteasome Inhibitor 1 x 100µl Proteasome Positive Control 1 vial Proteasome Substrate 1 x 100µl -
Research areas
Images
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Proteasome Activity measured in Jurkat lysate
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Proteasome Activity measured in HeLa lysate
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Proteasome Activity measured in cell lysates showing activity (mU) per 1 mln cells.
Samples with the concentration of 3.3e6 cells/mL were used. 50 μl of each sample was used.
Datasheets and documents
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SDS download
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Datasheet download
References (69)
ab107921 has been referenced in 69 publications.
- Wang W et al. Analysis of a degron-containing reporter protein GFP-CL1 reveals a role for SUMO1 in cytosolic protein quality control. J Biol Chem 299:102851 (2023). PubMed: 36587767
- Cheng KC et al. Early Aß42 Exposure Causes Learning Impairment in Later Life. Aging Dis 13:868-883 (2022). PubMed: 35656119
- Chen F et al. Comparative maternal protein profiling of mouse biparental and uniparental embryos. Gigascience 11:N/A (2022). PubMed: 36056732
- Nagendra AH et al. Sodium fluoride induces skeletal muscle atrophy via changes in mitochondrial and sarcomeric proteomes. PLoS One 17:e0279261 (2022). PubMed: 36548359
- Deng X et al. CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1. J Cell Biol 221:N/A (2022). PubMed: 34726688