Human Predicted to work with:
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
Synthetic peptide: DYIDAEHLGD FHRTYKNSEE LRSRIVSGII TPIHEQWEKA NVSSPHREFP, corresponding to a region within internal sequence amino acids 110-159 of Human Proteasome Inhibitor subunit 1 PI3 1 (NP_006805).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionPlays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
Sequence similaritiesBelongs to the proteasome inhibitor PI31 family.