Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab3893)

Overview

  • Product name
    Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody
    See all Proteasome Inhibitor subunit 1 PI3 1 primary antibodies
  • Description
    Goat polyclonal to Proteasome Inhibitor subunit 1 PI3 1
  • Specificity
    This antibody is expected to recognise isoform 1 (NP_006805, NP_848693) only, not isoform 2 (NP_848694).
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: DHLPPPGYDDMYL, corresponding to C terminal amino acids 259-271 of Human Proteasome Inhibitor subunit 1 PI3 1.

  • Positive control
    • Human Kidney lysate.
  • General notes
    Principal Names – PSMF1; PI31; proteasome (prosome, macropain) inhibitor subunit 1 (PI31); proteasome inhibitor hP131 subunit Official Gene Symbol - PSMF1 GenBank Accession Number – NP_006805; NP_848693 LocusLink ID - 9491 (human); 228769 (mouse).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/32000.
    WB: Use at a concentration of 1 - 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody images

    • ab3893 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    References for Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab3893)

    ab3893 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    Specification
    MEF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 17 2017

    Thank you for sending the details of your protocol and the picture of your blot. I really think that the background bands are due to the secondary. What appears to be happening is that the secondary is binding additional proteins and reacting with the ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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