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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 27 kDa.
1/100 - 1/500.
1/100 - 1/200.
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Sequence similarities Belongs to the peptidase T1A family.
Cellular localization Cytoplasm. Nucleus.
Information by UniProt
27 kDa prosomal protein antibody
Macropain iota chain antibody
Anti-Proteasome subunit alpha type 6 antibody images
Western blot - Proteasome subunit alpha type 6 antibody (ab97563)
All lanes : Anti-Proteasome subunit alpha type 6 antibody (ab97563) at 1/1000 dilution Lane 1 : 293T whole cell lysate Lane 2 : A431 whole cell lysate Lane 3 : H1299 whole cell lysate Lane 4 : HeLa whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size : 27 kDa
Immunocytochemistry/ Immunofluorescence - Proteasome subunit alpha type 6 antibody (ab97563)
ab97563, at a 1/200 dilution, staining Proteasome subunit alpha type 6 in paraformaldehyde-fixed A431 cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteasome subunit alpha type 6 antibody (ab97563)
ab97563, at a 1/100 dilution, staining Proteasome subunit alpha type 6 in paraffin-embedded H1299 xenograft by Immunohistochemical analysis.
References for Anti-Proteasome subunit alpha type 6 antibody (ab97563)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"