Anti-Proteasome subunit alpha type 6 antibody (ab97563)

Overview

  • Product nameAnti-Proteasome subunit alpha type 6 antibody
    See all Proteasome subunit alpha type 6 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome subunit alpha type 6
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to Human Proteasome subunit alpha type 6 aa 57-244.
    Database link: P60900

  • Positive control
    • WB: 293T, A431, H1299, HeLa, HepG2 and MOLT4 cell lysates IF: A431 cells IHC-P: H1299 xenograft

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
  • Sequence similaritiesBelongs to the peptidase T1A family.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 27 kDa prosomal protein antibody
    • IOTA antibody
    • Macropain iota chain antibody
    • Macropain subunit iota antibody
    • MGC22756 antibody
    • MGC2333 antibody
    • MGC23846 antibody
    • Multicatalytic endopeptidase complex iota chain antibody
    • p27K antibody
    • PROS 27 antibody
    • PROS-27 antibody
    • PROS27 antibody
    • Prosomal P27K protein antibody
    • Proteasome (prosome macropain) subunit alpha type 6 antibody
    • Proteasome iota chain antibody
    • Proteasome subunit alpha type 6 antibody
    • Proteasome subunit alpha type-6 antibody
    • Proteasome subunit iota antibody
    • PSA6_HUMAN antibody
    • PSMA 6 antibody
    • PSMA6 antibody
    see all

Anti-Proteasome subunit alpha type 6 antibody images

  • All lanes : Anti-Proteasome subunit alpha type 6 antibody (ab97563) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 27 kDa
  • ab97563, at a 1/200 dilution, staining Proteasome subunit alpha type 6 in paraformaldehyde-fixed A431 cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
  • ab97563, at a 1/100 dilution, staining Proteasome subunit alpha type 6 in paraffin-embedded H1299 xenograft by Immunohistochemical analysis.

References for Anti-Proteasome subunit alpha type 6 antibody (ab97563)

ab97563 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"