Anti-Proteasome subunit alpha type 6 antibody [EPR5835] (ab109377)


  • Product nameAnti-Proteasome subunit alpha type 6 antibody [EPR5835]
    See all Proteasome subunit alpha type 6 primary antibodies
  • Description
    Rabbit monoclonal [EPR5835] to Proteasome subunit alpha type 6
  • Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 6 aa 200-300 (C terminal).
    Database link: P60900

  • Positive control
    • WB: HepG2, 293T, HeLa and Jurkat cell lysates IHC-P: Human colon tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab109377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
ICC 1/250 - 1/500.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
    • Sequence similaritiesBelongs to the peptidase T1A family.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 27 kDa prosomal protein antibody
      • IOTA antibody
      • Macropain iota chain antibody
      • Macropain subunit iota antibody
      • MGC22756 antibody
      • MGC2333 antibody
      • MGC23846 antibody
      • Multicatalytic endopeptidase complex iota chain antibody
      • p27K antibody
      • PROS 27 antibody
      • PROS-27 antibody
      • PROS27 antibody
      • Prosomal P27K protein antibody
      • Proteasome (prosome macropain) subunit alpha type 6 antibody
      • Proteasome iota chain antibody
      • Proteasome subunit alpha type 6 antibody
      • Proteasome subunit alpha type-6 antibody
      • Proteasome subunit iota antibody
      • PSA6_HUMAN antibody
      • PSMA 6 antibody
      • PSMA6 antibody
      see all

    Anti-Proteasome subunit alpha type 6 antibody [EPR5835] images

    • All lanes : Anti-Proteasome subunit alpha type 6 antibody [EPR5835] (ab109377) at 1/50000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 27 kDa
      Observed band size : 27 kDa
    • ab109377, at a 1/250 dilution, staining Proteasome 20S alpha 6 in paraffin embedded Human colon tissue by Immunohistochemistry.

    References for Anti-Proteasome subunit alpha type 6 antibody [EPR5835] (ab109377)

    This product has been referenced in:
    • Ando R  et al. Decreased proteasomal activity causes photoreceptor degeneration in mice. Invest Ophthalmol Vis Sci 55:4682-90 (2014). Mouse . Read more (PubMed: 24994871) »

    See 1 Publication for this product

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