Anti-Proteasome subunit beta type 2 antibody [MCP165] (ab22650)

Overview

  • Product nameAnti-Proteasome subunit beta type 2 antibody [MCP165]
    See all Proteasome subunit beta type 2 primary antibodies
  • Description
    Mouse monoclonal [MCP165] to Proteasome subunit beta type 2
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein corresponding to Human Proteasome subunit beta type 2.
    Database link: P49721

  • Positive control
    • HeLa cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 5mM Sodium Azide
    Constituents: 20mM Tris HCl, 100mM Sodium chloride, pH 8
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPartially purified by ion-exchange from cell culture supernatant.
  • ClonalityMonoclonal
  • Clone numberMCP165
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 28 kDa.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.
    • Sequence similaritiesBelongs to the peptidase T1B family.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • HC7 I antibody
      • Macropain subunit C7 I antibody
      • Macropain subunit C7-I antibody
      • Multicatalytic endopeptidase complex subunit C7 1 antibody
      • Multicatalytic endopeptidase complex subunit C7 I antibody
      • Multicatalytic endopeptidase complex subunit C7-I antibody
      • Proteasome (prosome, macropain) subunit beta type 2 antibody
      • Proteasome beta 2 subunit antibody
      • Proteasome component C7 I antibody
      • Proteasome component C7-I antibody
      • Proteasome subunit beta type-2 antibody
      • PSB2_HUMAN antibody
      • Psmb2 antibody
      see all

    Anti-Proteasome subunit beta type 2 antibody [MCP165] images

    • Lane 1 : Protein Marker IV
      Lanes 2 - 7 : Anti-Proteasome subunit beta type 2 antibody [MCP165] (ab22650) at 1/1000 dilution

      Lane 1 : Protein Marker IV
      Lane 2 : Protein extract from ciliary body of diseased human donor at 2 µg
      Lane 3 : Protein extract from ciliary body of normal human donor at 2 µg
      Lane 4 : Protein extract from ciliary body of diseased human donor at 2 µg
      Lane 5 : Protein extract from ciliary body of normal human donor at 2 µg
      Lane 6 : Protein extract from ciliary body of diseased human donor at 2 µg
      Lane 7 : Protein extract from ciliary body of normal human donor at 2 µg

      Secondary
      Lanes 2 - 7 : HRP conjugated goat anti mouse antibody


      Observed band size : 27 kDa (why is the actual band size different from the predicted?)

      This image is courtesy of an Abreview submitted by Dr Matthias Zenkel

      See Abreview

    • IHC image of ab22650 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22650, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Overlay histogram showing HeLa cells stained with ab22650 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22650, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References for Anti-Proteasome subunit beta type 2 antibody [MCP165] (ab22650)

    This product has been referenced in:
    • Leite NC  et al. Protein malnutrition potentiates the amplifying pathway of insulin secretion in adult obese mice. Sci Rep 6:33464 (2016). WB ; Mouse . Read more (PubMed: 27633083) »
    • Ren H  et al. Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E(-/-) Mice. Sci Rep 5:15730 (2015). Read more (PubMed: 26508670) »

    See all 4 Publications for this product

    Product Wall


    I can confirm that this particular antibody is sourced externally from a collaborator. Unfortunately we are not able to release the immunogen sequence as they consider this proprietary information.

    They have confirmed that this antibody r...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (ciliary body)
    Loading amount 2 µg
    Specification ciliary body
    Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Dr. Matthias Zenkel

    Verified customer

    Submitted May 04 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"