Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (ab22650)
Key features and details
- Mouse monoclonal [MCP165] to Proteasome subunit beta type 2/PSMB2
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165]
See all Proteasome subunit beta type 2/PSMB2 primary antibodies -
Description
Mouse monoclonal [MCP165] to Proteasome subunit beta type 2/PSMB2 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human Proteasome subunit beta type 2/PSMB2. Dinitrophenylated proteasomes
Database link: P49721 -
Positive control
- WB: HeLa cell lysate; human ciliary body lysate. IHC-P: Human lymphoid tissue. Flow cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.032% Sodium azide
Constituents: 0.316% Tris HCl, 0.58% Sodium chloride -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MCP165 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
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Notes |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity. -
Sequence similarities
Belongs to the peptidase T1B family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5690 Human
- Omim: 602175 Human
- SwissProt: P49721 Human
- Unigene: 471441 Human
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Alternative names
- HC7 I antibody
- Macropain subunit C7 I antibody
- Macropain subunit C7-I antibody
see all
Images
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Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (ab22650) at 1/1000 dilution + HeLa cell lysate
Predicted band size: 23 kDa -
Lane 1 : Protein Marker IV
Lanes 2-7 : Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (ab22650) at 1/1000 dilution
Lane 1 : Protein Marker IV
Lanes 2 & 4 & 6 : Protein extract from ciliary body of diseased human donor at 2 µg
Lanes 3 & 5 & 7 : Protein extract from ciliary body of normal human donor at 2 µg
Secondary
Lanes 2-7 : HRP conjugated goat anti mouse antibody
Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
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IHC image of ab22650 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22650, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Overlay histogram showing HeLa cells stained with ab22650 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22650, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab22650 has been referenced in 4 publications.
- Leite NC et al. Protein malnutrition potentiates the amplifying pathway of insulin secretion in adult obese mice. Sci Rep 6:33464 (2016). WB ; Mouse . PubMed: 27633083
- Ren H et al. Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E(-/-) Mice. Sci Rep 5:15730 (2015). PubMed: 26508670
- Arellano-Garcia ME et al. Interferon-? induces immunoproteasomes and the presentation of MHC I-associated peptides on human salivary gland cells. PLoS One 9:e102878 (2014). WB ; Human . PubMed: 25102056
- Durgeau A et al. Different expression levels of the TAP peptide transporter lead to recognition of different antigenic peptides by tumor-specific CTL. J Immunol 187:5532-9 (2011). WB ; Human . PubMed: 22025554