The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3 µg/ml.
Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal macrophage proteasome (By similarity). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
Sequence similaritiesBelongs to the peptidase T1B family.
Proteasome subunit beta type 4 antibody (ab55628) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.
Western blot - Proteasome subunit beta type 4 antibody (ab55628)
Western blot against tagged recombinant protein immunogen using ab55628 Proteasome subunit beta type 4 antibody at 1ug/ml. Predicted band size of immunogen is 55 kDa
Flow Cytometry-Anti-Proteasome subunit beta type 4 antibody(ab55628)
Overlay histogram showing HeLa cells stained with ab55628 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55628, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-Proteasome subunit beta type 4 antibody (ab55628)
This product has been referenced in:
Thaker NG et al. Identification of survival genes in human glioblastoma cells by small interfering RNA screening. Mol Pharmacol76:1246-55 (2009).
Read more (PubMed: 19783622) »
Catlow K et al. Identification of a gastrin response element in the vesicular monoamine transporter type 2 promoter and requirement of 20 S proteasome subunits for transcriptional activity. J Biol Chem282:17069-77 (2007).
Read more (PubMed: 17442673) »