Protein A Agarose (High Affinity) (ab193255)

Overview

  • Product name
    Protein A Agarose (High Affinity)
  • Sample type
    Cell culture supernatant, Serum, Cell culture media, Ascites Fluid
  • Product overview

    Contents:

    Supplied as 50% slurry in 20% Ethanol.

     

    Features:
    High binding capacity = Binding of IgG = 36 mg human or rabbit IgG/mL Protein A Agarose.
    Minimal leaching of the ligand
    Flow Rate Tested* = 2.89 mL/min.
    *Test conditions: = Calculations based on the time required to pass 18 mL of water through 2 mL settled beads (column diameter 1.5 cm).
    Maximum Flow Rate** = 1800 cm/hr; minimum leaching of recombinant Protein A.
    **NOTE = the highest flow that beads withstand for 1 minute, without collapsing and the pressure reaching 1 MPa.

    Usage = Reusable for up to 10 times without significant loss of binding capacity.

    These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.

    Store beads at 4°C.

    The beads may be damaged above 40°C.

    DO NOT FREEZE.

    Wash beads 3 times with 3x bead volume of desired buffer before use.

     

    Applications:
    - Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
    - Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.

  • Notes

    Abcam's Protein A Agarose (High Affinity) beads are specially prepared for high IgG binding by covalently coupling recombinant Protein A to 6% cross-linked agarose beads, the most popular resin for protein affinity purification methods. Protein A is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A compared to standard Protein A agarose beads. The IgG binding capacity of Protein A Agarose (High Affinity) is = 36 mg human or rabbit IgG per mL of wet beads. The Protein A Agarose (High Affinity) beads display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation.

  • Tested applications
    Suitable for: IP, Purificationmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab193255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.

Purification Use at an assay dependent concentration.

Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.

References

ab193255 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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