Overview

  • Product name
  • Description
    Chicken polyclonal to Protein A
  • Host species
    Chicken
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Full length protein corresponding to Protein A.
    Database link: P02976

  • Positive control
    • Protein A

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 56 kDa.

Target

  • Relevance
    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localization
    Secreted; cell wall.
  • Alternative names
    • gG-binding protein A antibody
    • Immunoglobulin G binding protein A antibody
    • SPA antibody
    • Staphylococcal protein A antibody

Images

  • Lane 1 : Anti-Protein A antibody (ab183882) at 1/10000 dilution
    Lane 2 : GFP antibody at 1/10000 dilution

    All lanes : Protein A, 0.1 ng

    Predicted band size: 56 kDa



    Easy blocker  was used to block interaction of protein A with secondary antibody.

References

ab183882 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab183882.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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