Overview

  • Product name
    Anti-Protein A antibody (Biotin)
    See all Protein A primary antibodies
  • Description
    Chicken polyclonal to Protein A (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Immunogen

    Recombinant full length protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.1% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    After multiple immunizations in Freund’s adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein A agarose and conjugated with biotin.
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.2 - 2 µg/ml.
WB 1/1000 - 1/5000.
ELISA 1/10000 - 1/50000.

Target

  • Relevance
    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localization
    Secreted; cell wall.
  • Alternative names
    • gG-binding protein A antibody
    • Immunoglobulin G binding protein A antibody
    • SPA antibody
    • Staphylococcal protein A antibody

References

This product has been referenced in:
  • Gilbert FB  et al. Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system. Vet Res 44:40 (2013). WB ; Staphylococcus aureus . Read more (PubMed: 23758654) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting Abcam regarding ab18598 and ab19483.


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The conjugated antibodies ab18596 and ab18598 are prepared from the unconjugated ab19483. The formulations differ (for instance, there is no azide in the HRP conjugate buffer) but the antibody is the same for all...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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